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Literature summary extracted from

  • Doan, T.N.; Prabhu, P.; Kim, J.K.; Ahn, Y.J.; Natarajan, S.; Kang, L.W.; Park, G.T.; Lim, S.B.; Lee, J.K.
    Crystallization and preliminary X-ray crystallographic analysis of L-rhamnose isomerase with a novel high thermostability from Bacillus halodurans (2010), Acta Crystallogr. Sect. F, 66, 677-680.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.1.14 expressed in Escherichia coli BL21(DE3) cells Halalkalibacterium halodurans

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.3.1.14 hanging drop vapor diffusion method, using 10% (w/v) PEG 8000, 0.1 M HEPES pH 6.0 and 0.2 M sodium acetate Halalkalibacterium halodurans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.1.14 48000
-
 x * 48000, SDS-PAGE Halalkalibacterium halodurans

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.14 Halalkalibacterium halodurans
-
-
-
5.3.1.14 Halalkalibacterium halodurans ATCC BAA-125
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.1.14 Ni-NTA column chromatography Halalkalibacterium halodurans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.14 L-Rhamnose i.e 6-deoxy-L-mannose Halalkalibacterium halodurans L-Rhamnulose i.e 6-deoxy-L-fructose r
5.3.1.14 L-Rhamnose i.e 6-deoxy-L-mannose Halalkalibacterium halodurans ATCC BAA-125 L-Rhamnulose i.e 6-deoxy-L-fructose r

Subunits

EC Number Subunits Comment Organism
5.3.1.14 ? x * 48000, SDS-PAGE Halalkalibacterium halodurans

Synonyms

EC Number Synonyms Comment Organism
5.3.1.14 L-RhI
-
 Halalkalibacterium halodurans