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Literature summary extracted from

  • Brown, S.A.; Whiteley, M.
    Characterization of the L-lactate dehydrogenase from Aggregatibacter actinomycetemcomitans (2009), PLoS ONE, 4, e7864.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.27 gene AA02769 or lctD, DNA and amino acid sequence determination and analysis, subcloning in Escherichia coli DH5alpha, expression as His6-tagged protein in Escherichia coli BL21(DE3) Aggregatibacter actinomycetemcomitans

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.27 additional information construction of an insertion mutant of gene AA02769, inactivation of AA02769 eliminates the ability of the organism to grow on L-lactate, but not on D-glucose Aggregatibacter actinomycetemcomitans

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.27 additional information the Aggregatibacter actinomycetemcomitans L-lactate dehydrogenase, unlike homologous enzymes, is not feedback inhibited by pyruvate, pyruvate is a poor inhibitor of L-lactate dehydrogenase activity Aggregatibacter actinomycetemcomitans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.27 0.152
-
 L-lactate pH not specified in the publication, temperature not specified in the publication Aggregatibacter actinomycetemcomitans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.27 42000
-
 x * 42000, recombinant His6-tagged LctD, SDS-PAGE Aggregatibacter actinomycetemcomitans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.27 L-lactate + NAD+ Aggregatibacter actinomycetemcomitans no activity with D-lactate pyruvate + NADH + H+
-
 r
1.1.1.27 L-lactate + NAD+ Aggregatibacter actinomycetemcomitans HK1651 and VT1169 no activity with D-lactate pyruvate + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.27 Aggregatibacter actinomycetemcomitans
-
 a Gram-negative opportunistic pathogen found exclusively in the mammalian oral cavity in the space between the gums and the teeth known as the gingival crevice, gene AA02769
-
1.1.1.27 Aggregatibacter actinomycetemcomitans HK1651 and VT1169
-
 a Gram-negative opportunistic pathogen found exclusively in the mammalian oral cavity in the space between the gums and the teeth known as the gingival crevice, gene AA02769
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.27 native enzyme, and recombinant His6-tagged enzyme by nickel affinity chromatography from Escherichia coli BL21(DE3) Aggregatibacter actinomycetemcomitans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.27 L-lactate + NAD+ no activity with D-lactate Aggregatibacter actinomycetemcomitans pyruvate + NADH + H+
-
 r
1.1.1.27 L-lactate + NAD+ no activity with D-lactate, activity of recombinant His6-tagged LctD is determined in the presence of the electron carriers 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide and phenazine methosulfate Aggregatibacter actinomycetemcomitans pyruvate + NADH + H+
-
 r
1.1.1.27 L-lactate + NAD+ no activity with D-lactate Aggregatibacter actinomycetemcomitans HK1651 and VT1169 pyruvate + NADH + H+
-
 r
1.1.1.27 L-lactate + NAD+ no activity with D-lactate, activity of recombinant His6-tagged LctD is determined in the presence of the electron carriers 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide and phenazine methosulfate Aggregatibacter actinomycetemcomitans HK1651 and VT1169 pyruvate + NADH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.27 ? x * 42000, recombinant His6-tagged LctD, SDS-PAGE Aggregatibacter actinomycetemcomitans

Synonyms

EC Number Synonyms Comment Organism
1.1.1.27 LctD
-
 Aggregatibacter actinomycetemcomitans

General Information

EC Number General Information Comment Organism
1.1.1.27 metabolism the enzyme catalyzes the first step in L-lactate catabolism Aggregatibacter actinomycetemcomitans