Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Mueller, I.B.; Bergmann, B.; Groves, M.R.; Couto, I.; Amaral, L.; Begley, T.P.; Walter, R.D.; Wrenger, C.
    The vitamin B1 metabolism of Staphylococcus aureus is controlled at enzymatic and transcriptional levels (2009), PLoS ONE, 4, e7656.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.99.2 expressed in Escherichia coli BLR (DE3) cells Staphylococcus aureus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.99.2 0.256
-
 thiamine apparent value, in 100 mM Tris-HCl, pH 7.5, at 37°C Staphylococcus aureus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.99.2 82900
-
 static light scattering Staphylococcus aureus

Organism

EC Number Organism UniProt Comment Textmining
3.5.99.2 Staphylococcus aureus
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.99.2 0.0046
-
 in 100 mM Tris-HCl, pH 7.5, at 37°C Staphylococcus aureus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.99.2 additional information TenA does not accept TMP and TPP as substrates Staphylococcus aureus ?
-
 ?
3.5.99.2 thiamine + H2O
-
 Staphylococcus aureus 4-amino-5-hydroxymethyl-2-methylpyrimidine + 5-(2-hydroxyethyl)-4-methylthiazole
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.99.2 trimer in vitro Staphylococcus aureus

Synonyms

EC Number Synonyms Comment Organism
3.5.99.2 TenA
-
 Staphylococcus aureus
3.5.99.2 thiaminase II
-
 Staphylococcus aureus
3.5.99.2 thiaminase type II
-
 Staphylococcus aureus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.99.2 0.0017
-
 thiamine in 100 mM Tris-HCl, pH 7.5, at 37°C Staphylococcus aureus

General Information

EC Number General Information Comment Organism
3.5.99.2 metabolism thiaminase is involved in vitamin B1 metabolism Staphylococcus aureus