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Literature summary extracted from

  • Ernst, M.; de Padua, R.M.; Herl, V.; Mueller-Uri, F.; Kreis, W.
    Expression of 3beta-HSD and P5betaR, genes respectively coding for Delta5-3beta-hydroxysteroid dehydrogenase and progesterone 5beta-reductase, in leaves and cell cultures of Digitalis lanata EHRH (2010), Planta Med., 76, 923-927.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.51 gene 3bhsd, quantitative real-time PCR expression analysis Digitalis lanata

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.51 5beta-pregnan-3,20-dione + NADPH + H+ Digitalis lanata
-
 5beta-pregnane-3beta-ol-20-one + NADP+
-
 ?
1.1.1.51 5beta-pregnan-3,20-dione + NADPH + H+ Digitalis lanata EHRH
-
 5beta-pregnane-3beta-ol-20-one + NADP+
-
 ?
1.1.1.51 pregnenolone + NADPH + H+ Digitalis lanata
-
 isoprogesterone + NADP+
-
 ?
1.1.1.51 pregnenolone + NADPH + H+ Digitalis lanata EHRH
-
 isoprogesterone + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.51 Digitalis lanata Q93Y47
-
-
1.1.1.145 Digitalis lanata Q93Y47
-
-
1.1.1.145 Digitalis lanata EHRH Q93Y47
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.51 cell suspension culture
-
 Digitalis lanata
-
1.1.1.51 K3OHD cell
-
 Digitalis lanata
-
1.1.1.51 leaf
-
 Digitalis lanata
-
1.1.1.51 additional information the enzyme is also expressed in cardenolide-free tissues of cardenolide-containing plants Digitalis lanata
-
1.1.1.145 cell suspension culture cardenolide-free cell suspension culture Digitalis lanata
-
1.1.1.145 leaf cardenolide-accumulating leaf Digitalis lanata
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.51 5beta-pregnan-3,20-dione + NADPH + H+
-
 Digitalis lanata 5beta-pregnane-3beta-ol-20-one + NADP+
-
 ?
1.1.1.51 5beta-pregnan-3,20-dione + NADPH + H+
-
 Digitalis lanata EHRH 5beta-pregnane-3beta-ol-20-one + NADP+
-
 ?
1.1.1.51 pregnenolone + NADPH + H+
-
 Digitalis lanata isoprogesterone + NADP+
-
 ?
1.1.1.51 pregnenolone + NADPH + H+
-
 Digitalis lanata EHRH isoprogesterone + NADP+
-
 ?
1.1.1.145 additional information enzyme catalyzes at least two reactions in the cardenolide pathway, i.e. dehydrogenation of pregnenolone to isoprogesterone and the reduction of 5beta-pregnane-3,20-dione to 5beta-pregnan-3beta-ol-20-one. Recombinant enzyme displays 3alpha-, 3beta- and 17beta-dehydrogenase as well as 3/17-keto reductase activities Digitalis lanata ?
-
 ?
1.1.1.145 additional information enzyme catalyzes at least two reactions in the cardenolide pathway, i.e. dehydrogenation of pregnenolone to isoprogesterone and the reduction of 5beta-pregnane-3,20-dione to 5beta-pregnan-3beta-ol-20-one. Recombinant enzyme displays 3alpha-, 3beta- and 17beta-dehydrogenase as well as 3/17-keto reductase activities Digitalis lanata EHRH ?
-
 ?
1.1.1.145 pregnenolone + NAD+
-
 Digitalis lanata isoprogesterone + NADH + H+
-
 ?
1.1.1.145 pregnenolone + NAD+
-
 Digitalis lanata EHRH isoprogesterone + NADH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.51 3beta-HSD
-
 Digitalis lanata
1.1.1.51 3beta-hydroxysteroid dehydrogenase
-
 Digitalis lanata

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.51 NADPH
-
 Digitalis lanata

General Information

EC Number General Information Comment Organism
1.1.1.51 physiological function key enzyme in cardenolide biosynthesis Digitalis lanata