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Literature summary extracted from

  • Fincato, P.; Moschou, P.N.; Spedaletti, V.; Tavazza, R.; Angelini, R.; Federico, R.; Roubelakis-Angelakis, K.A.; Tavladoraki, P.
    Functional diversity inside the Arabidopsis polyamine oxidase gene family (2010), J. Exp. Bot., 6, 1155-1168.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.3.16 0.02
-
thermospermine pH 8.0, temperature not specified in the publication Arabidopsis thaliana
1.5.3.16 0.047
-
spermine pH 7.5, temperature not specified in the publication Arabidopsis thaliana
1.5.3.16 0.12
-
spermine pH 8.0, temperature not specified in the publication Arabidopsis thaliana
1.5.3.16 0.47
-
N1-acetylspermine pH 8.0, temperature not specified in the publication Arabidopsis thaliana
1.5.3.16 0.9
-
norspermine pH 8.0, temperature not specified in the publication Arabidopsis thaliana
1.5.3.17 0.045
-
norspermine pH 7.5, temperature not specified in the publication Arabidopsis thaliana
1.5.3.17 0.05
-
thermospermine pH 7.5, temperature not specified in the publication Arabidopsis thaliana
1.5.3.17 0.233
-
N1-acetylspermine pH 7.5, temperature not specified in the publication Arabidopsis thaliana
1.5.3.17 0.27
-
spermine pH 7.5, temperature not specified in the publication Arabidopsis thaliana
1.5.3.17 0.274
-
spermidine pH 7.5, temperature not specified in the publication Arabidopsis thaliana
1.5.3.17 0.409
-
spermidine pH 7.5, temperature not specified in the publication Arabidopsis thaliana
1.5.3.17 0.58
-
spermine pH 7.5, temperature not specified in the publication Arabidopsis thaliana
1.5.3.17 2
-
N1-acetylspermine pH 7.5, temperature not specified in the publication Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
1.5.3.16 Arabidopsis thaliana Q8H191 isoform PAO4
-
1.5.3.16 Arabidopsis thaliana Q9FNA2 isoform PAO1
-
1.5.3.17 Arabidopsis thaliana Q9LYT1 isoform PAO3
-
1.5.3.17 Arabidopsis thaliana Q9SKX5 isoform PAO2
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.3.16 additional information no substrate: spermidine. Comparative study of the catalytic properties of recombinant AtPAO1, AtPAO2, AtPAO3, and AtPAO4. All four enzymes strongly resemble their mammalian counterparts, being able to oxidize the common polyamines Spd and/or Spm through a polyamine backconversion pathway Arabidopsis thaliana ?
-
?
1.5.3.16 additional information spermidine is oxidized with very low efficiency. Comparative study of the catalytic properties of recombinant AtPAO1, AtPAO2, AtPAO3, and AtPAO4. All four enzymes strongly resemble their mammalian counterparts, being able to oxidize the common polyamines Spd and/or Spm through a polyamine backconversion pathway Arabidopsis thaliana ?
-
?
1.5.3.16 N1-acetylspermine + O2 + H2O
-
Arabidopsis thaliana spermidine + 3-acetaminopropanal + H2O2
-
?
1.5.3.16 norspermine + O2 + H2O
-
Arabidopsis thaliana ? + H2O2
-
?
1.5.3.16 spermine + O2 + H2O
-
Arabidopsis thaliana spermidine + aminopropanal + H2O2
-
?
1.5.3.16 thermospermine + O2 + H2O
-
Arabidopsis thaliana ? + H2O2
-
?
1.5.3.17 additional information comparative study of the catalytic properties of recombinant AtPAO1, AtPAO2, AtPAO3, and AtPAO4. All four enzymes strongly resemble their mammalian counterparts, being able to oxidize the common polyamines Spd and/or Spm through a polyamine backconversion pathway Arabidopsis thaliana ?
-
?
1.5.3.17 N1-acetylspermine + O2 + H2O
-
Arabidopsis thaliana spermidine + 3-acetaminopropanal + H2O2
-
?
1.5.3.17 norspermine + O2 + H2O
-
Arabidopsis thaliana ? + H2O2
-
?
1.5.3.17 spermidine + O2 + H2O
-
Arabidopsis thaliana putrescine + 3-aminopropanal + H2O2
-
?
1.5.3.17 spermine + O2 + H2O
-
Arabidopsis thaliana spermidine + aminopropanal + H2O2
-
?
1.5.3.17 thermospermine + O2 + H2O
-
Arabidopsis thaliana ? + H2O2
-
?

Synonyms

EC Number Synonyms Comment Organism
1.5.3.16 PAO4
-
Arabidopsis thaliana
1.5.3.17 PAO2
-
Arabidopsis thaliana
1.5.3.17 PAO3
-
Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.3.16 0.014
-
N1-acetylspermine pH 7.5, temperature not specified in the publication Arabidopsis thaliana
1.5.3.16 0.1
-
thermospermine pH 7.5, temperature not specified in the publication Arabidopsis thaliana
1.5.3.16 0.2
-
N1-acetylspermine pH 8.0, temperature not specified in the publication Arabidopsis thaliana
1.5.3.16 0.45
-
norspermine pH 7.5, temperature not specified in the publication Arabidopsis thaliana
1.5.3.16 2.5
-
spermine pH 8.0, temperature not specified in the publication Arabidopsis thaliana
1.5.3.16 4.6
-
spermine pH 7.5, temperature not specified in the publication Arabidopsis thaliana
1.5.3.16 5.7
-
thermospermine pH 8.0, temperature not specified in the publication Arabidopsis thaliana
1.5.3.16 6.9
-
norspermine pH 8.0, temperature not specified in the publication Arabidopsis thaliana
1.5.3.17 0.02
-
N1-acetylspermine pH 7.5, temperature not specified in the publication Arabidopsis thaliana
1.5.3.17 0.4
-
thermospermine pH 7.5, temperature not specified in the publication Arabidopsis thaliana
1.5.3.17 0.5
-
thermospermine pH 7.5, temperature not specified in the publication Arabidopsis thaliana
1.5.3.17 0.8
-
N1-acetylspermine pH 7.5, temperature not specified in the publication Arabidopsis thaliana
1.5.3.17 1.1
-
norspermine pH 7.5, temperature not specified in the publication Arabidopsis thaliana
1.5.3.17 1.7
-
spermine pH 7.5, temperature not specified in the publication Arabidopsis thaliana
1.5.3.17 2.9
-
norspermine pH 7.5, temperature not specified in the publication Arabidopsis thaliana
1.5.3.17 3.4
-
spermidine pH 7.5, temperature not specified in the publication Arabidopsis thaliana
1.5.3.17 4.2
-
spermine pH 7.5, temperature not specified in the publication Arabidopsis thaliana
1.5.3.17 4.6
-
spermidine pH 7.5, temperature not specified in the publication Arabidopsis thaliana

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.5.3.16 0.43
-
N1-acetylspermine pH 8.0, temperature not specified in the publication Arabidopsis thaliana
1.5.3.16 7.7
-
norspermine pH 8.0, temperature not specified in the publication Arabidopsis thaliana
1.5.3.16 20.8
-
spermine pH 8.0, temperature not specified in the publication Arabidopsis thaliana
1.5.3.16 285
-
thermospermine pH 8.0, temperature not specified in the publication Arabidopsis thaliana
1.5.3.17 0.01
-
N1-acetylspermine pH 7.5, temperature not specified in the publication Arabidopsis thaliana
1.5.3.17 2.9
-
spermine pH 7.5, temperature not specified in the publication Arabidopsis thaliana
1.5.3.17 3.4
-
N1-acetylspermine pH 7.5, temperature not specified in the publication Arabidopsis thaliana
1.5.3.17 10
-
thermospermine pH 7.5, temperature not specified in the publication Arabidopsis thaliana
1.5.3.17 11.2
-
spermidine pH 7.5, temperature not specified in the publication Arabidopsis thaliana
1.5.3.17 12.4
-
spermidine pH 7.5, temperature not specified in the publication Arabidopsis thaliana
1.5.3.17 15.6
-
spermine pH 7.5, temperature not specified in the publication Arabidopsis thaliana
1.5.3.17 24.4
-
norspermine pH 7.5, temperature not specified in the publication Arabidopsis thaliana