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Literature summary extracted from

  • Palmen, L.G.; Kvassman, J.O.
    Differential inhibition of homotrimeric dUTPases by the 3-azido derivative of dideoxy-UTP (2010), J. Enzyme Inhib. Med. Chem., 25, 146-150.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.23 expressed in Escherichia coli BL21(DE3)pLysS cells Escherichia coli
3.6.1.23 expressed in Escherichia coli BL21(DE3)pLysS cells Homo sapiens
3.6.1.23 expressed in Escherichia coli BL21(DE3)pLysS cells equine infectious anemia virus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.23 3'-azido-2',3'-dideoxy-UTP competitive inhibitor Escherichia coli
3.6.1.23 additional information the enzyme is not inhibited by 3'-azido-2',3'-dideoxy-UTP equine infectious anemia virus
3.6.1.23 additional information the enzyme is not inhibited by 3'-azido-2',3'-dideoxy-UTP Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.23 0.00017
-
 dUTP pH and temperature not specified in the publication Homo sapiens
3.6.1.23 0.00022
-
 dUTP pH and temperature not specified in the publication Escherichia coli
3.6.1.23 0.00096
-
 dUTP pH and temperature not specified in the publication equine infectious anemia virus

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.23 equine infectious anemia virus
-
-
-
3.6.1.23 Escherichia coli
-
-
-
3.6.1.23 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.23 phosphocellulose column chromatography Escherichia coli
3.6.1.23 phosphocellulose column chromatography Homo sapiens
3.6.1.23 phosphocellulose column chromatography equine infectious anemia virus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.23 dUTP + H2O
-
 Escherichia coli dUMP + diphosphate
-
 ?
3.6.1.23 dUTP + H2O
-
 Homo sapiens dUMP + diphosphate
-
 ?
3.6.1.23 dUTP + H2O
-
 equine infectious anemia virus dUMP + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.1.23 homotrimer
-
 Escherichia coli
3.6.1.23 homotrimer
-
 Homo sapiens
3.6.1.23 homotrimer
-
 equine infectious anemia virus

Synonyms

EC Number Synonyms Comment Organism
3.6.1.23 dUTPase
-
 Escherichia coli
3.6.1.23 dUTPase
-
 Homo sapiens
3.6.1.23 dUTPase
-
 equine infectious anemia virus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.6.1.23 0.0093
-
 3'-azido-2',3'-dideoxy-UTP 0.02 mM 3'-azido-2',3'-dideoxy-UTP in 0.05 mM bicine, with 5 mM MgCl2, pH and temperature not specified in the publication Escherichia coli