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Literature summary extracted from

  • Nabeta, K.; Fujita, M.; Komuro, K.; Katayama, K.; Takasawa, T.
    In vitro biosynthesis of cadinanes by cell-free extracts of cultured cells of Heteroscyphus planus (1997), J. Chem. Soc., 1, 2065-2070.
No PubMed abstract available

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.62 (2Z, 6E)-farnesyl diphosphate Heteroscyphus planus
-
 (-)-gamma-cadinene + diphosphate formation of gamma-cadinene involves a 1,3-hydride shift. (-)-gamma-Cadinene is directly formed from 2Z,6E-farnesyl diphosphate by intramolecular electrophilic attack of the primary carbocation on the C-10 position of farnesyl diphosphate. Second product: (+)-germacrene D ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.62 Heteroscyphus planus
-
-
-
4.2.3.63 Heteroscyphus planus
-
 bifunctional (+)-cubenene synthase and (+)-epicubenol synthase
-
4.2.3.64 Heteroscyphus planus
-
 bifunctional (+)-cubenene synthase and (+)-epicubenol synthase
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.3.62 partial Heteroscyphus planus
4.2.3.63
-
 Heteroscyphus planus
4.2.3.64
-
 Heteroscyphus planus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.3.62 268
-
 pH 7.5, 30°C Heteroscyphus planus
4.2.3.63 268
-
 pH not specified in the publication, temperature not specified in the publication Heteroscyphus planus
4.2.3.64 5433
-
 pH not specified in the publication, temperature not specified in the publication Heteroscyphus planus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.62 (2Z, 6E)-farnesyl diphosphate
-
 Heteroscyphus planus (-)-gamma-cadinene + diphosphate formation of gamma-cadinene involves a 1,3-hydride shift. (-)-gamma-Cadinene is directly formed from 2Z,6E-farnesyl diphosphate by intramolecular electrophilic attack of the primary carbocation on the C-10 position of farnesyl diphosphate. Second product: (+)-germacrene D ?
4.2.3.63 (2E,6E)-farnesyl diphosphate specific substrae Heteroscyphus planus (+)-cubenene + diphosphate (+)-cubenene synthase and (+)-epicubenol synthase are dual functions of the same enzyme ?
4.2.3.64 (2E,6E)-farnesyl diphosphate + H2O specific substrate Heteroscyphus planus (+)-epicubenol + diphosphate (+)-cubenene synthase and (+)-epicubenol synthase are dual functions of the same enzyme ?