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Literature summary extracted from

  • Courjean, O.; Mano, N.
    Recombinant glucose oxidase from Penicillium amagasakiense for efficient bioelectrochemical applications in physiological conditions (2011), J. Biotechnol., 151, 122-129.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.3.4 biotechnology GOX is the most widely used enzyme for the development of electrochemical glucose biosensors and biofuel cell in physiological conditions Aspergillus niger
1.1.3.4 biotechnology GOX is the most widely used enzyme for the development of electrochemical glucose biosensors and biofuel cell in physiological conditions Penicillium amagasakiense

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.3.4 expressed in Pichia pastoris strain GS115 Aspergillus niger
1.1.3.4 expressed in Pichia pastoris strain GS115 Penicillium amagasakiense

General Stability

EC Number General Stability Organism
1.1.3.4 the enzyme stability is not influenced by physiological concentration of sodium chloride (140 mM) Aspergillus niger
1.1.3.4 the enzyme stability is not influenced by physiological concentration of sodium chloride (140 mM) Penicillium amagasakiense

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.3.4 oxygen
-
 Aspergillus niger
1.1.3.4 oxygen
-
 Penicillium amagasakiense

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.4 0.0638
-
 ferrocinium-methanol recombinant enzyme yGOXpenag, pH 6.0, 50°C Penicillium amagasakiense
1.1.3.4 0.1107
-
 ferrocinium-methanol native enzyme, pH 6.5, 70°C Aspergillus niger
1.1.3.4 11.7
-
 beta-D-glucose recombinant enzyme yGOXpenag, using O2 as cosubstrate, pH 6.0, 50°C Penicillium amagasakiense
1.1.3.4 18.2
-
 beta-D-glucose recombinant enzyme yGOXpenag, using ferrocinium-methanol as cosubstrate, pH 6.0, 50°C Penicillium amagasakiense
1.1.3.4 50.3
-
 beta-D-glucose native enzyme, using O2 as cosubstrate, pH 6.5, 70°C Aspergillus niger
1.1.3.4 71.2
-
 beta-D-glucose native enzyme, using ferrocinium-methanol as cosubstrate, pH 6.5, 70°C Aspergillus niger

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.4 72000
-
 2 * 72000, recombinant enzyme, SDS-PAGE Penicillium amagasakiense
1.1.3.4 77000
-
 2 * 77000, native enzyme, SDS-PAGE Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.4 Aspergillus niger
-
-
-
1.1.3.4 Penicillium amagasakiense
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.1.3.4 glycoprotein
-
 Aspergillus niger
1.1.3.4 glycoprotein
-
 Penicillium amagasakiense

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.4 phenyl Sepharose column chromatography and Q Sepharose column chromatography Penicillium amagasakiense
1.1.3.4 phenyl Sepharose column chromatography, Q Sepharose column chromatography, gel filtration Aspergillus niger

Storage Stability

EC Number Storage Stability Organism
1.1.3.4 4°C, purified recombinant enzyme in 100 mM sodium phosphate, pH 5.1, several months, no loss of activity Aspergillus niger
1.1.3.4 4°C, purified recombinant enzyme in 100 mM sodium phosphate, pH 5.1, several months, no loss of activity Penicillium amagasakiense

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.4 beta-D-glucose + ferrocinium-methanol
-
 Aspergillus niger ?
-
 ?
1.1.3.4 beta-D-glucose + ferrocinium-methanol
-
 Penicillium amagasakiense ?
-
 ?
1.1.3.4 beta-D-glucose + O2 + H2O
-
 Aspergillus niger D-glucono-1,5-lactone + H2O2
-
 ?
1.1.3.4 beta-D-glucose + O2 + H2O
-
 Penicillium amagasakiense D-glucono-1,5-lactone + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.3.4 homodimer 2 * 72000, recombinant enzyme, SDS-PAGE Penicillium amagasakiense
1.1.3.4 homodimer 2 * 77000, native enzyme, SDS-PAGE Aspergillus niger

Synonyms

EC Number Synonyms Comment Organism
1.1.3.4 beta-D-glucose:oxygen 1-oxidoreductase
-
 Aspergillus niger
1.1.3.4 beta-D-glucose:oxygen 1-oxidoreductase
-
 Penicillium amagasakiense
1.1.3.4 GOX
-
 Aspergillus niger
1.1.3.4 GOX
-
 Penicillium amagasakiense
1.1.3.4 yGOXpenag recombinant enzyme Penicillium amagasakiense

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.3.4 50
-
 recombinant enzyme Penicillium amagasakiense
1.1.3.4 70
-
-
 Aspergillus niger

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.3.4 37
-
 at 37°C and in pH 7.5 buffer, the half life of the native enzyme is 48 h Aspergillus niger
1.1.3.4 37
-
 at 37°C and in pH 7.5 buffer, the half life of the recombinant enzyme yGOXpenag is 6 h Penicillium amagasakiense
1.1.3.4 50
-
 at pH 6.0 and at 50°C, the half life of the enzyme is about 20 h, thermal denaturation is observed above 50°C Penicillium amagasakiense
1.1.3.4 50 60 at pH 6.0 and at 50°C, the half life of the enzyme is about 20 h, thermal denaturation is observed above 60°C Aspergillus niger

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.3.4 1695
-
 beta-D-glucose recombinant enzyme yGOXpenag, using ferrocinium-methanol as cosubstrate, pH 6.0, 50°C Penicillium amagasakiense
1.1.3.4 1808
-
 beta-D-glucose recombinant enzyme yGOXpenag, using O2 as cosubstrate, pH 6.0, 50°C Penicillium amagasakiense
1.1.3.4 1890
-
 beta-D-glucose native enzyme, using O2 as cosubstrate, pH 6.5, 70°C Aspergillus niger
1.1.3.4 1938
-
 beta-D-glucose native enzyme, using ferrocinium-methanol as cosubstrate, pH 6.5, 70°C Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.4 6
-
 under O2 and Ar at 37°C, recombinant enzyme yGOXpenag Penicillium amagasakiense
1.1.3.4 6.5
-
 native enzyme Aspergillus niger

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.3.4 5 7.5 under O2 and Ar at 37°C, recombinant enzyme yGOXpenag Penicillium amagasakiense
1.1.3.4 5.5 9 80% of the maximal activity is observed in the pH 5.5-9.0 range Aspergillus niger

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.3.4 6
-
 at pH 6.0 and at 50°C, the half life of the enzyme is about 20 h Aspergillus niger
1.1.3.4 6
-
 at pH 6.0 and at 50°C, the half life of the enzyme is about 20 h Penicillium amagasakiense

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.4 FAD
-
 Aspergillus niger
1.1.3.4 FAD
-
 Penicillium amagasakiense

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.3.4 0.017
-
 ferrocinium-methanol native enzyme, pH 6.5, 70°C Aspergillus niger
1.1.3.4 0.027
-
 ferrocinium-methanol recombinant enzyme yGOXpenag, pH 6.0, 50°C Penicillium amagasakiense
1.1.3.4 27
-
 beta-D-glucose native enzyme, using ferrocinium-methanol as cosubstrate, pH 6.5, 70°C Aspergillus niger
1.1.3.4 38
-
 beta-D-glucose native enzyme, using O2 as cosubstrate, pH 6.5, 70°C Aspergillus niger
1.1.3.4 93
-
 beta-D-glucose recombinant enzyme yGOXpenag, using ferrocinium-methanol as cosubstrate, pH 6.0, 50°C Penicillium amagasakiense
1.1.3.4 155
-
 beta-D-glucose recombinant enzyme yGOXpenag, using O2 as cosubstrate, pH 6.0, 50°C Penicillium amagasakiense