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Literature summary extracted from

  • Murakami, C.; Ohmae, E.; Tate, S.; Gekko, K.; Nakasone, K.; Kato, C.
    Cloning and characterization of dihydrofolate reductases from deep-sea bacteria (2010), J. Biochem., 147, 591-599.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.1.3 expressed in Escherichia coli Escherichia coli
1.5.1.3 expressed in Escherichia coli Shewanella violacea
1.5.1.3 expressed in Escherichia coli Photobacterium profundum
1.5.1.3 expressed in Escherichia coli Moritella yayanosii
1.5.1.3 expressed in Escherichia coli Moritella japonica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.3 0.001
-
 dihydrofolate pH 7.0, 25°C, under atmospheric pressure Escherichia coli
1.5.1.3 0.0018
-
 dihydrofolate pH 7.0, 25°C, under atmospheric pressure Moritella yayanosii
1.5.1.3 0.0019
-
 NADPH pH 7.0, 25°C, under atmospheric pressure Escherichia coli
1.5.1.3 0.0019
-
 dihydrofolate pH 7.0, 25°C, under atmospheric pressure Shewanella violacea
1.5.1.3 0.0034
-
 NADPH pH 7.0, 25°C, under atmospheric pressure Shewanella violacea
1.5.1.3 0.0062
-
 dihydrofolate pH 7.0, 25°C, under atmospheric pressure Photobacterium profundum
1.5.1.3 0.0198
-
 NADPH pH 7.0, 25°C, under atmospheric pressure Photobacterium profundum
1.5.1.3 0.0584
-
 NADPH pH 7.0, 25°C, under atmospheric pressure Moritella japonica
1.5.1.3 0.101
-
 NADPH pH 7.0, 25°C, under atmospheric pressure Moritella yayanosii
1.5.1.3 0.238
-
 dihydrofolate pH 7.0, 25°C, under atmospheric pressure Moritella japonica

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.3 Escherichia coli
-
-
-
1.5.1.3 Moritella japonica D1MX72
-
-
1.5.1.3 Moritella yayanosii D1MX71
-
-
1.5.1.3 Photobacterium profundum D1MX70
-
-
1.5.1.3 Shewanella violacea D1MYR1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.3 using affinity chromatography Escherichia coli
1.5.1.3 using affinity chromatography Shewanella violacea
1.5.1.3 using affinity chromatography Photobacterium profundum
1.5.1.3 using affinity chromatography Moritella yayanosii
1.5.1.3 using affinity chromatography Moritella japonica

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.3 additional information
-
 ecDHFR shows a monotonous decrease of its activity with increasing pressure, down to 50% at 250 MPa Escherichia coli
1.5.1.3 additional information
-
 mjDHFR loses its activity significantly even in the low-pressure region and almost completely above 100 MPa, which may be related to its low substrate-binding ability Moritella japonica
1.5.1.3 additional information
-
 ppDHFR shows a monotonous decrease of its activity with increasing pressure, down to 20% at 250 MPa Photobacterium profundum
1.5.1.3 additional information
-
 the activity is invariant below 75 MPa and then gradually decreases with increasing pressure down to 30% at 250 MPa Moritella yayanosii
1.5.1.3 additional information
-
 the enzymatic activity of svDHFR increases with pressure to 110% at around 75 MPa and then decreases to 60% at 250 MPa Shewanella violacea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.3 dihydrofolate + NADPH + H+
-
 Escherichia coli tetrahydrofolate + NADP+
-
 ?
1.5.1.3 dihydrofolate + NADPH + H+
-
 Shewanella violacea tetrahydrofolate + NADP+
-
 ?
1.5.1.3 dihydrofolate + NADPH + H+
-
 Photobacterium profundum tetrahydrofolate + NADP+
-
 ?
1.5.1.3 dihydrofolate + NADPH + H+
-
 Moritella yayanosii tetrahydrofolate + NADP+
-
 ?
1.5.1.3 dihydrofolate + NADPH + H+
-
 Moritella japonica tetrahydrofolate + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.5.1.3 dihydrofolate reductase
-
 Escherichia coli
1.5.1.3 dihydrofolate reductase
-
 Shewanella violacea
1.5.1.3 dihydrofolate reductase
-
 Photobacterium profundum
1.5.1.3 dihydrofolate reductase
-
 Moritella yayanosii
1.5.1.3 dihydrofolate reductase
-
 Moritella japonica
1.5.1.3 ecDHFR
-
 Escherichia coli
1.5.1.3 mjDHFR
-
 Moritella japonica
1.5.1.3 myDHFR
-
 Moritella yayanosii
1.5.1.3 ppDHFR
-
 Photobacterium profundum
1.5.1.3 svDHFR
-
 Shewanella violacea

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.1.3 25
-
 assay at Escherichia coli
1.5.1.3 25
-
 assay at Shewanella violacea
1.5.1.3 25
-
 assay at Photobacterium profundum
1.5.1.3 25
-
 assay at Moritella yayanosii
1.5.1.3 25
-
 assay at Moritella japonica
1.5.1.3 25
-
 optimal temperature Moritella yayanosii
1.5.1.3 30
-
 optimal temperature Moritella japonica
1.5.1.3 40
-
 optimal temperature Shewanella violacea
1.5.1.3 53
-
 optimal temperature Photobacterium profundum
1.5.1.3 57
-
 optimal temperature Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.1.3 18.4
-
 dihydrofolate pH 7.0, 25°C, under atmospheric pressure Escherichia coli
1.5.1.3 19.2
-
 NADPH pH 7.0, 25°C, under atmospheric pressure Escherichia coli
1.5.1.3 80.3
-
 NADPH pH 7.0, 25°C, under atmospheric pressure Moritella yayanosii
1.5.1.3 83.8
-
 dihydrofolate pH 7.0, 25°C, under atmospheric pressure Moritella yayanosii
1.5.1.3 86.8
-
 NADPH pH 7.0, 25°C, under atmospheric pressure Shewanella violacea
1.5.1.3 90
-
 dihydrofolate pH 7.0, 25°C, under atmospheric pressure Shewanella violacea
1.5.1.3 109
-
 NADPH pH 7.0, 25°C, under atmospheric pressure Moritella japonica
1.5.1.3 144
-
 dihydrofolate pH 7.0, 25°C, under atmospheric pressure Moritella japonica
1.5.1.3 150.5
-
 NADPH pH 7.0, 25°C, under atmospheric pressure Photobacterium profundum
1.5.1.3 156
-
 dihydrofolate pH 7.0, 25°C, under atmospheric pressure Photobacterium profundum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.3 6.5
-
 optimal pH Moritella japonica
1.5.1.3 7
-
 assay at Escherichia coli
1.5.1.3 7
-
 assay at Shewanella violacea
1.5.1.3 7
-
 assay at Photobacterium profundum
1.5.1.3 7
-
 assay at Moritella yayanosii
1.5.1.3 7
-
 assay at Moritella japonica
1.5.1.3 7
-
 optimal pH Shewanella violacea
1.5.1.3 7
-
 optimal pH Photobacterium profundum
1.5.1.3 7.5
-
 optimal pH Moritella yayanosii
1.5.1.3 8
-
 optimal pH Escherichia coli

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.5.1.3 600
-
 dihydrofolate pH 7.0, 25°C, under atmospheric pressure Moritella japonica
1.5.1.3 800
-
 NADPH pH 7.0, 25°C, under atmospheric pressure Moritella yayanosii
1.5.1.3 1900
-
 NADPH pH 7.0, 25°C, under atmospheric pressure Moritella japonica
1.5.1.3 7600
-
 NADPH pH 7.0, 25°C, under atmospheric pressure Photobacterium profundum
1.5.1.3 10100
-
 NADPH pH 7.0, 25°C, under atmospheric pressure Escherichia coli
1.5.1.3 16700
-
 dihydrofolate pH 7.0, 25°C, under atmospheric pressure Escherichia coli
1.5.1.3 25200
-
 dihydrofolate pH 7.0, 25°C, under atmospheric pressure Photobacterium profundum
1.5.1.3 25500
-
 NADPH pH 7.0, 25°C, under atmospheric pressure Shewanella violacea
1.5.1.3 46600
-
 dihydrofolate pH 7.0, 25°C, under atmospheric pressure Moritella yayanosii
1.5.1.3 47400
-
 dihydrofolate pH 7.0, 25°C, under atmospheric pressure Shewanella violacea