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Literature summary extracted from

  • Li, Z.C.; Chen, L.H.; Yu, X.J.; Hu, Y.H.; Song, K.K.; Zhou, X.W.; Chen, Q.X.
    Inhibition kinetics of chlorobenzaldehyde thiosemicarbazones on mushroom tyrosinase (2010), J. Agric. Food Chem., 58, 12537-12540.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.18.1 2-chlorobenzaldehyde thiosemicarbazone exhibits significant inhibitory potency on both monophenolase activity and diphenolase activity of tyrosinase, reversible noncompetitive inhibitor Agaricus bisporus
1.14.18.1 4-chlorobenzaldehyde thiosemicarbazone exhibits significant inhibitory potency on both monophenolase activity and diphenolase activity of tyrosinase, reversible mixed-type inhibitor Agaricus bisporus

Organism

EC Number Organism UniProt Comment Textmining
1.14.18.1 Agaricus bisporus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.18.1 L-DOPA + O2 diphenolase activity Agaricus bisporus dopaquinone + H2O
-
 ?
1.14.18.1 L-tyrosine + O2 monophenolase activity Agaricus bisporus L-DOPA + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.18.1 monophenol, o-diphenol:oxygen oxidoreductase
-
 Agaricus bisporus
1.14.18.1 tyrosinase
-
 Agaricus bisporus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.14.18.1 0.0012
-
 2-chlorobenzaldehyde thiosemicarbazone in 50 mM Na2HPO4-NaH2PO4 buffer (pH 6.8), at 30°C Agaricus bisporus
1.14.18.1 0.00125
-
 4-chlorobenzaldehyde thiosemicarbazone in 5 0mM Na2HPO4-NaH2PO4 buffer (pH 6.8), at 30°C Agaricus bisporus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.14.18.1 0.00122
-
 using L-Dopa as substrate, in 50 mM Na2HPO4-NaH2PO4 buffer (pH 6.8), at 30°C Agaricus bisporus 2-chlorobenzaldehyde thiosemicarbazone
1.14.18.1 0.00182
-
 using L-Dopa as substrate, in 50 mM Na2HPO4-NaH2PO4 buffer (pH 6.8), at 30°C Agaricus bisporus 4-chlorobenzaldehyde thiosemicarbazone
1.14.18.1 0.0067
-
 using L-tyrosine as substrate, in 50 mM Na2HPO4-NaH2PO4 buffer (pH 6.8), at 30°C Agaricus bisporus 4-chlorobenzaldehyde thiosemicarbazone
1.14.18.1 0.0154
-
 using L-tyrosine as substrate, in 50 mM Na2HPO4-NaH2PO4 buffer (pH 6.8), at 30°C Agaricus bisporus 2-chlorobenzaldehyde thiosemicarbazone