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Literature summary extracted from

  • Rocha, J.; Popescu, A.O.; Sa-Correia, I.; Fialho, A.M.; Frazao, C.
    Cloning, expression, purification, crystallization and preliminary crystallographic studies of BceC, a UDP-glucose dehydrogenase from Burkholderia cepacia IST408 (2010), Acta Crystallogr. Sect. F, 66, 269-271.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.22 gene bceC, DNA and amino acid sequence determination and analysis, expression in Escherichia coli Burkholderia cepacia

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.22 purified recombinant His6-tagged BceC, 0.001 ml of protein solution containing 5 mg/ml protein in 25 mM Tris-HCl, pH 8.3, 50 mM NaCl, 2.5 mM DTT, 0.25 mM UDP-GlcA, and 0.5 mM NAD+, is mixed with 0.001 ml of precipitant solution containing 200 mM ammonium sulfate, 100 mM sodium acetate, pH 4.5, 11% w/v PEG 4000, and 50 mM NaF, method optimiization, X-ray diffraction structure determination and analysis at 2.09 A resolution, molecular replacement Burkholderia cepacia

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.22 52600
-
 x * 52600, recombinant His6-tagged BceC, SDS-PAGE Burkholderia cepacia

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.22 UDP-glucose + 2 NAD+ + H2O Burkholderia cepacia
-
 UDP-glucuronic acid + 2 NADH + 2 H+
-
 ?
1.1.1.22 UDP-glucose + 2 NAD+ + H2O Burkholderia cepacia IST408
-
 UDP-glucuronic acid + 2 NADH + 2 H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.22 Burkholderia cepacia
-
 gene bceC
-
1.1.1.22 Burkholderia cepacia IST408
-
 gene bceC
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.22 recombinant His6-tagged BceC from Escherichia coli by affinity chromatography Burkholderia cepacia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.22 UDP-glucose + 2 NAD+ + H2O
-
 Burkholderia cepacia UDP-glucuronic acid + 2 NADH + 2 H+
-
 ?
1.1.1.22 UDP-glucose + 2 NAD+ + H2O
-
 Burkholderia cepacia IST408 UDP-glucuronic acid + 2 NADH + 2 H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.22 ? x * 52600, recombinant His6-tagged BceC, SDS-PAGE Burkholderia cepacia

Synonyms

EC Number Synonyms Comment Organism
1.1.1.22 BceC
-
 Burkholderia cepacia
1.1.1.22 UDP-glucose dehydrogenase
-
 Burkholderia cepacia
1.1.1.22 uridine-5'-diphosphoglucose dehydrogenase
-
 Burkholderia cepacia

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.22 NAD+
-
 Burkholderia cepacia