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Literature summary extracted from

  • Cedervall, P.E.; Hooper, A.B.; Wilmot, C.M.
    Crystallization and preliminary X-ray crystallographic analysis of a new crystal form of hydroxylamine oxidoreductase from Nitrosomonas europaea (2009), Acta Crystallogr. Sect. F, 65, 1296-1298.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.7.2.6 microbatch-under-oil method, using 0.1 M potassium nitrate, 0.1 M MES pH 7.5 and 46% (v/v) PEG 400 Nitrosomonas europaea
1.7.2.6 using 0.1 M potassium nitrate, 0.1 M MES pH 7.5 and 46% (w/v) PEG 400 Nitrosomonas europaea
1.7.2.8 microbatch-under-oil method, using 0.1 M potassium nitrate, 0.1 M MES pH 7.5 and 46%(v/v) PEG 400 Nitrosomonas europaea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.2.6 67100
-
 3 * 67100 Nitrosomonas europaea
1.7.2.8 67100
-
 3 * 67100, calculated from amino acid sequence Nitrosomonas europaea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.2.6 hydroxylamine + ferricytochrome c + H2O Nitrosomonas europaea
-
 nitrite + ferrocytochrome c + H+
-
 ?
1.7.2.8 hydroxylamine + 2 acceptor + H2O Nitrosomonas europaea
-
 nitrite + 2 reduced acceptor + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.7.2.6 Nitrosomonas europaea
-
-
-
1.7.2.8 Nitrosomonas europaea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.2.6 ammonium sulfate precipitation, Sepharose CL-6B column chromatography, DEAE-Sephacel column chromatography, and Sephadex G-100 gel filtration Nitrosomonas europaea
1.7.2.6 ammonium sulfate precipitation, Sepharose CL-6B column chromatography, DEAE-Sephacel gel filtration, and Sephadex GĀ—100 gel filtration Nitrosomonas europaea
1.7.2.8 ammonium sulfate precipitation, Sepharose CL-6B column chromatography, Sephadex G-100 gel filtration, and DEAE-Sephacel column chromatography Nitrosomonas europaea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.2.6 hydroxylamine + ferricytochrome c + H2O
-
 Nitrosomonas europaea nitrite + ferrocytochrome c + H+
-
 ?
1.7.2.8 hydroxylamine + 2 acceptor + H2O
-
 Nitrosomonas europaea nitrite + 2 reduced acceptor + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.7.2.6 homotrimer x-ray crystallography Nitrosomonas europaea
1.7.2.6 homotrimer 3 * 67100 Nitrosomonas europaea
1.7.2.8 homotrimer 3 * 67100, calculated from amino acid sequence Nitrosomonas europaea

Synonyms

EC Number Synonyms Comment Organism
1.7.2.6 HAO
-
 Nitrosomonas europaea
1.7.2.6 hydroxylamine oxidoreductase
-
 Nitrosomonas europaea
1.7.2.8 HAO
-
 Nitrosomonas europaea
1.7.2.8 hydroxylamine oxidoreductase
-
 Nitrosomonas europaea

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.2.6 heme each monomer contains seven c-type hemes and one heme P460 Nitrosomonas europaea
1.7.2.6 heme each subunit contains seven c-type hemes and one heme P460 Nitrosomonas europaea
1.7.2.8 heme each monomer contains seven c-type hemes and one heme P460 Nitrosomonas europaea