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Literature summary extracted from

  • Park, H.; Lee, H.; Ro, Y.T.; Kim, Y.M.
    Identification and functional characterization of a gene for the methanol: N,N'-dimethyl-4-nitrosoaniline oxidoreductase from Mycobacterium sp. strain JC1 (DSM 3803) (2010), Microbiology, 156, 463-471.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.99.37
-
 Mycobacterium sp.
1.2.98.1
-
 Mycobacterium sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.99.37 46000
-
 x * 46000, SDS-PAGE, x * 46223, calculated Mycobacterium sp.
1.1.99.37 46223
-
 x * 46000, SDS-PAGE, x * 46223, calculated Mycobacterium sp.

Organism

EC Number Organism UniProt Comment Textmining
1.1.99.37 Mycobacterium sp. C5MRT8
-
-
1.1.99.37 Mycobacterium sp. DSM 3803 / JC1 C5MRT8
-
-
1.2.98.1 Mycobacterium sp. C5MRT8 bifunctional enzyme. In addition to dismutase activity, enzyme oxidizes ethanol and formaldehyde as well as methanol
-
1.2.98.1 Mycobacterium sp. DSM 3803 C5MRT8 bifunctional enzyme. In addition to dismutase activity, enzyme oxidizes ethanol and formaldehyde as well as methanol
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.99.37
-
 Mycobacterium sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.99.37 0.35
-
 substrate formaldehyde Mycobacterium sp.
1.1.99.37 0.37
-
 substrate ethanol Mycobacterium sp.
1.1.99.37 0.48
-
 substrate N,N-dimethyl-4-nitrosoaniline Mycobacterium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.99.37 ethanol + N,N-dimethyl-4-nitrosoaniline
-
 Mycobacterium sp. acetaldehyde + 4-(hydroxylamino)-N,N-dimethylaniline
-
 r
1.1.99.37 ethanol + N,N-dimethyl-4-nitrosoaniline
-
 Mycobacterium sp. DSM 3803 / JC1 acetaldehyde + 4-(hydroxylamino)-N,N-dimethylaniline
-
 r
1.1.99.37 ethanol + N,N-dimethyl-4-nitrosoaniline
-
 Mycobacterium sp. DSM 3803 acetaldehyde + 4-(hydroxylamino)-N,N-dimethylaniline
-
 r
1.1.99.37 formaldehyde + 4-(hydroxylamino)-N,N-dimethylaniline
-
 Mycobacterium sp. methanol + N,N-dimethyl-4-nitrosoaniline
-
 r
1.1.99.37 formaldehyde + 4-(hydroxylamino)-N,N-dimethylaniline
-
 Mycobacterium sp. DSM 3803 / JC1 methanol + N,N-dimethyl-4-nitrosoaniline
-
 r
1.1.99.37 formaldehyde + 4-(hydroxylamino)-N,N-dimethylaniline
-
 Mycobacterium sp. DSM 3803 methanol + N,N-dimethyl-4-nitrosoaniline
-
 r
1.1.99.37 methanol + N,N-dimethyl-4-nitrosoaniline
-
 Mycobacterium sp. formaldehyde + 4-(hydroxylamino)-N,N-dimethylaniline
-
 r
1.1.99.37 methanol + N,N-dimethyl-4-nitrosoaniline
-
 Mycobacterium sp. DSM 3803 / JC1 formaldehyde + 4-(hydroxylamino)-N,N-dimethylaniline
-
 r
1.1.99.37 methanol + N,N-dimethyl-4-nitrosoaniline
-
 Mycobacterium sp. DSM 3803 formaldehyde + 4-(hydroxylamino)-N,N-dimethylaniline
-
 r
1.2.98.1 2 formaldehyde
-
 Mycobacterium sp. formate + methanol
-
 ?
1.2.98.1 2 formaldehyde
-
 Mycobacterium sp. DSM 3803 / JC1 formate + methanol
-
 ?
1.2.98.1 2 formaldehyde
-
 Mycobacterium sp. DSM 3803 formate + methanol
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.99.37 ? x * 46000, SDS-PAGE, x * 46223, calculated Mycobacterium sp.

Synonyms

EC Number Synonyms Comment Organism
1.1.99.37 MDO
-
 Mycobacterium sp.
1.1.99.37 methanol:N,N-dimethyl-4-nitrosoaniline oxidoreductase
-
 Mycobacterium sp.
1.2.98.1 N,N9-dimethyl-4-nitrosoaniline oxidoreductase
-
 Mycobacterium sp.

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.99.37 Mycobacterium sp. calculated
-
 5.4

Expression

EC Number Organism Comment Expression
1.1.99.37 Mycobacterium sp. transcription of methanol:N,N-dimethyl-4-nitrosoaniline oxidoreductase is upregulated in cells growing on methanol compared with cells growing on glucose up
1.2.98.1 Mycobacterium sp. transcription is upregulated in cells growing on methanol up

General Information

EC Number General Information Comment Organism
1.1.99.37 physiological function an enzyme-deficient mutant strain does not grow with methanol as a sole source of carbon and energy Mycobacterium sp.
1.2.98.1 physiological function an emzyme-deficient mutant cannot grow on methanol as sole source of carbon and energy Mycobacterium sp.