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Literature summary extracted from

  • Madrid, E.; Corchete, P.
    Silymarin secretion and its elicitation by methyl jasmonate in cell cultures of Silybum marianum is mediated by phospholipase D-phosphatidic acid (2010), J. Exp. Bot., 61, 747-754.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.4.4 mastoparan a PLD activity stimulator Silybum marianum
3.1.4.4 methyl {(1S,2S)-3-oxo-2-[(2Z)-pent-2-en-1-yl]cyclopentyl}acetate increases PLD activity Silybum marianum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.4 additional information treatment with iso-, sec- or tert-butanol has no effect on silymarin production Silybum marianum
3.1.4.4 n-butanol inhibits phosphatidic acid production by PLD, prevents silymarin elicitation by methyljasmonate or mastoparan, and also impedes its release in non-elicited cultures. Exogenous addition of phosphatidic acid reverses the inhibitory action of nBuOH, both in control and methyljasmonate-treated cultures Silybum marianum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.4.4 additional information Silybum marianum silymarin secretion and its elicitation by methyl jasmonate in cell cultures of Silybum marianum is mediated by phospholipase D-phosphatidic acid, overview ?
-
 ?
3.1.4.4 phosphatidylcholine + H2O Silybum marianum phosphatidic acid activates the production of and promotes accumulation of silymarin, overview choline + phosphatidic acid
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 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.4 Silybum marianum
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.4 1-hexadecanoyl-2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)aminohexanoyl]-sn-glycero-3-phosphocholine + H2O fluorescent substrate Silybum marianum ?
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 ?
3.1.4.4 additional information silymarin secretion and its elicitation by methyl jasmonate in cell cultures of Silybum marianum is mediated by phospholipase D-phosphatidic acid, overview Silybum marianum ?
-
 ?
3.1.4.4 phosphatidylcholine + H2O
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 Silybum marianum choline + phosphatidic acid
-
 ?
3.1.4.4 phosphatidylcholine + H2O phosphatidic acid activates the production of and promotes accumulation of silymarin, overview Silybum marianum choline + phosphatidic acid
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.4.4 PLD
-
 Silybum marianum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.4.4 30
-
 assay at Silybum marianum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.4 6.5
-
 assay at Silybum marianum

General Information

EC Number General Information Comment Organism
3.1.4.4 physiological function phospholipase D cleaves phospholipids into phosphatidic acid and free-head groups such as choline Silybum marianum