EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.20 | isopentenyl diphosphate + cis-1,4-polyisoprene | Hevea brasiliensis | 50 mM phosphate buffer, pH 7.5, 40 mM 2-mercaptoethanol, 40 mM KF, 8 mM MgCl2 + substrates, 30°C, 70% incorporation small rubber particles, 50% incorporation with FL-latex, 13% with washed small particles, 4% with highly purified washed small particles, with farnesyl diphosphate or dimethylallyl diphosphate as allylic initiators 70% incorporation, similar as for small rubber particles without allylic initiators | diphosphate + a poly-cis-polyprenyl diphosphate longer by one C5 unit | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.20 | Hevea brasiliensis | - |
rubber tree clone RRIM 600 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.5.1.20 | centrifugation to separate small rubber particles from the lower layer, dilution with 0.01 M phosphate buffer, pH 7.0 to 30% dry rubber content, washed particles are produced by washing with 0.25 M Tris-HCl, pH 8.0, with 1% Tween-20 and 5 mM 2-mercaptoethanol, applied to a Ultrogel AcA-44 column, centrifuged and diluted to 15% dry rubber content, highly purified small particles are produced by further centrifugation with 0.5% Triton X-100, exchange by buffer and centrifugation, cream phase diluted to 15% dry rubber content | Hevea brasiliensis |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.5.1.20 | additional information | - |
2.3 microM isopentenyl diphosphate incorporation/g dry rubber/4 h for small rubber particles, 0.7 microM isopentenyl diphosphate incorporation/g dry rubber/4 h for purified and washed small rubber particles, almost no isopentenyl diphosphate incorporation/g dry rubber/4 h for washed and highly purified small rubber particles, in vitro, 50 mM phosphate buffer, pH 7.5, 40 mM 2-mercaptoethanol, 40 mM KF, 8 mM MgCl2 + substrates, 30°C, small rubber particle activity decreases with increasing dimethylallyl diphosphate concentration, dimethylallyl diphosphate slightly increases activity in washed small particles not in a dose-dependent manner | Hevea brasiliensis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.20 | isopentenyl diphosphate + cis-1,4-polyisoprene | 50 mM phosphate buffer, pH 7.5, 40 mM 2-mercaptoethanol, 40 mM KF, 8 mM MgCl2 + substrates, 30°C, 70% incorporation small rubber particles, 50% incorporation with FL-latex, 13% with washed small particles, 4% with highly purified washed small particles, with farnesyl diphosphate or dimethylallyl diphosphate as allylic initiators 70% incorporation, similar as for small rubber particles without allylic initiators | Hevea brasiliensis | diphosphate + a poly-cis-polyprenyl diphosphate longer by one C5 unit | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.5.1.20 | cis-prenyltransferase | - |
Hevea brasiliensis |
2.5.1.20 | rubber transferase | - |
Hevea brasiliensis |