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Literature summary extracted from
- Screening, purification, and identification of the enzyme producing N-(L-alpha-L-aspartyl)-L-phenylalanine methyl ester from l-isoasparagine and L-phenylalanine methyl ester (2009), J. Biosci. Bioeng., 108, 190-193.
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.13.21 | 25000 | - |
SDS-PAGE | Escherichia coli |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.13.21 | Cedecea lapagei | - |
- |
- |
| 3.4.13.21 | Cedecea lapagei TPU 5750 | - |
- |
- |
| 3.4.13.21 | Escherichia coli | - |
- |
- |
| 3.4.13.21 | Escherichia coli TPU 6303 | - |
- |
- |
| 3.4.13.21 | Hafnia alvei | - |
- |
- |
| 3.4.13.21 | Hafnia alvei TPU 6440 | - |
- |
- |
| 3.4.13.21 | Klebsiella aerogenes | - |
- |
- |
| 3.4.13.21 | Klebsiella aerogenes TPU 6151 | - |
- |
- |
| 3.4.13.21 | Morganella morganii | - |
- |
- |
| 3.4.13.21 | Morganella morganii TPU 6501 | - |
- |
- |
| 3.4.13.21 | Rahnella aquatilis | - |
- |
- |
| 3.4.13.21 | Rahnella aquatilis TPU 5901 | - |
- |
- |
| 3.4.13.21 | Raoultella planticola | - |
- |
- |
| 3.4.13.21 | Raoultella planticola TPU 6501 | - |
- |
- |
| 3.4.13.21 | Serratia marcescens | - |
- |
- |
| 3.4.13.21 | Serratia marcescens TPU 7303 | - |
- |
- |
| 3.4.13.21 | Yersinia aldovae | - |
- |
- |
| 3.4.13.21 | Yersinia aldovae TPU 7650 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.13.21 | yield of 1.2% after 5-20% gradient acrylamide slab gel | Escherichia coli |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.4.13.21 | 3.39 | - |
purified enzyme | Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.13.21 | L-isoasparagine + L-phenylalanine methyl ester | assay at 30°C, pH 10.0 | Escherichia coli | N-(L-alpha-L-aspartyl)-L-phenylalanine methyl ester + ? | - |
? |  |
| 3.4.13.21 | L-isoasparagine + L-phenylalanine methyl ester | assay at 30°C, pH 10.0 | Klebsiella aerogenes | N-(L-alpha-L-aspartyl)-L-phenylalanine methyl ester + ? | - |
? | |
| 3.4.13.21 | L-isoasparagine + L-phenylalanine methyl ester | assay at 30°C, pH 10.0 | Serratia marcescens | N-(L-alpha-L-aspartyl)-L-phenylalanine methyl ester + ? | - |
? | |
| 3.4.13.21 | L-isoasparagine + L-phenylalanine methyl ester | assay at 30°C, pH 10.0 | Morganella morganii | N-(L-alpha-L-aspartyl)-L-phenylalanine methyl ester + ? | - |
? | |
| 3.4.13.21 | L-isoasparagine + L-phenylalanine methyl ester | assay at 30°C, pH 10.0 | Raoultella planticola | N-(L-alpha-L-aspartyl)-L-phenylalanine methyl ester + ? | - |
? | |
| 3.4.13.21 | L-isoasparagine + L-phenylalanine methyl ester | assay at 30°C, pH 10.0 | Hafnia alvei | N-(L-alpha-L-aspartyl)-L-phenylalanine methyl ester + ? | - |
? | |
| 3.4.13.21 | L-isoasparagine + L-phenylalanine methyl ester | assay at 30°C, pH 10.0 | Rahnella aquatilis | N-(L-alpha-L-aspartyl)-L-phenylalanine methyl ester + ? | - |
? | |
| 3.4.13.21 | L-isoasparagine + L-phenylalanine methyl ester | assay at 30°C, pH 10.0 | Yersinia aldovae | N-(L-alpha-L-aspartyl)-L-phenylalanine methyl ester + ? | - |
? | |
| 3.4.13.21 | L-isoasparagine + L-phenylalanine methyl ester | assay at 30°C, pH 10.0 | Cedecea lapagei | N-(L-alpha-L-aspartyl)-L-phenylalanine methyl ester + ? | - |
? | |
| 3.4.13.21 | L-isoasparagine + L-phenylalanine methyl ester | assay at 30°C, pH 10.0 | Klebsiella aerogenes TPU 6151 | N-(L-alpha-L-aspartyl)-L-phenylalanine methyl ester + ? | - |
? | |
| 3.4.13.21 | L-isoasparagine + L-phenylalanine methyl ester | assay at 30°C, pH 10.0 | Yersinia aldovae TPU 7650 | N-(L-alpha-L-aspartyl)-L-phenylalanine methyl ester + ? | - |
? | |
| 3.4.13.21 | L-isoasparagine + L-phenylalanine methyl ester | assay at 30°C, pH 10.0 | Rahnella aquatilis TPU 5901 | N-(L-alpha-L-aspartyl)-L-phenylalanine methyl ester + ? | - |
? | |
| 3.4.13.21 | L-isoasparagine + L-phenylalanine methyl ester | assay at 30°C, pH 10.0 | Cedecea lapagei TPU 5750 | N-(L-alpha-L-aspartyl)-L-phenylalanine methyl ester + ? | - |
? | |
| 3.4.13.21 | L-isoasparagine + L-phenylalanine methyl ester | assay at 30°C, pH 10.0 | Escherichia coli TPU 6303 | N-(L-alpha-L-aspartyl)-L-phenylalanine methyl ester + ? | - |
? | |
| 3.4.13.21 | L-isoasparagine + L-phenylalanine methyl ester | assay at 30°C, pH 10.0 | Morganella morganii TPU 6501 | N-(L-alpha-L-aspartyl)-L-phenylalanine methyl ester + ? | - |
? | |
| 3.4.13.21 | L-isoasparagine + L-phenylalanine methyl ester | assay at 30°C, pH 10.0 | Serratia marcescens TPU 7303 | N-(L-alpha-L-aspartyl)-L-phenylalanine methyl ester + ? | - |
? | |
| 3.4.13.21 | L-isoasparagine + L-phenylalanine methyl ester | assay at 30°C, pH 10.0 | Hafnia alvei TPU 6440 | N-(L-alpha-L-aspartyl)-L-phenylalanine methyl ester + ? | - |
? | |
| 3.4.13.21 | L-isoasparagine + L-phenylalanine methyl ester | assay at 30°C, pH 10.0 | Raoultella planticola TPU 6501 | N-(L-alpha-L-aspartyl)-L-phenylalanine methyl ester + ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.13.21 | dipeptidase E | - |
Escherichia coli |
| 3.4.13.21 | dipeptidase E | - |
Klebsiella aerogenes |
| 3.4.13.21 | dipeptidase E | - |
Serratia marcescens |
| 3.4.13.21 | dipeptidase E | - |
Morganella morganii |
| 3.4.13.21 | dipeptidase E | - |
Raoultella planticola |
| 3.4.13.21 | dipeptidase E | - |
Hafnia alvei |
| 3.4.13.21 | dipeptidase E | - |
Rahnella aquatilis |
| 3.4.13.21 | dipeptidase E | - |
Yersinia aldovae |
| 3.4.13.21 | dipeptidase E | - |
Cedecea lapagei |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.13.21 | 30 | - |
assay at | Escherichia coli |
| 3.4.13.21 | 30 | - |
assay at | Klebsiella aerogenes |
| 3.4.13.21 | 30 | - |
assay at | Serratia marcescens |
| 3.4.13.21 | 30 | - |
assay at | Morganella morganii |
| 3.4.13.21 | 30 | - |
assay at | Raoultella planticola |
| 3.4.13.21 | 30 | - |
assay at | Hafnia alvei |
| 3.4.13.21 | 30 | - |
assay at | Rahnella aquatilis |
| 3.4.13.21 | 30 | - |
assay at | Yersinia aldovae |
| 3.4.13.21 | 30 | - |
assay at | Cedecea lapagei |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.13.21 | 10 | - |
assay at | Escherichia coli |
| 3.4.13.21 | 10 | - |
assay at | Klebsiella aerogenes |
| 3.4.13.21 | 10 | - |
assay at | Serratia marcescens |
| 3.4.13.21 | 10 | - |
assay at | Morganella morganii |
| 3.4.13.21 | 10 | - |
assay at | Raoultella planticola |
| 3.4.13.21 | 10 | - |
assay at | Hafnia alvei |
| 3.4.13.21 | 10 | - |
assay at | Rahnella aquatilis |
| 3.4.13.21 | 10 | - |
assay at | Yersinia aldovae |
| 3.4.13.21 | 10 | - |
assay at | Cedecea lapagei |
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