Literature summary extracted from
Hidaka, M.; Fushinobu, S.; Honda, Y.; Wakagi, T.; Shoun, H.; Kitaoka, M.
Structural explanation for the acquisition of glycosynthase activity (2010), J. Biochem., 147, 237-244.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
3.2.1.156 |
expression of wild-type and mutant enzymes in Escherichia coli strain BL21 |
Halalkalibacterium halodurans |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
3.2.1.156 |
mutants of reducing-end xylose-releasing exooligoxylanase with glycosynthase activity, X-ray diffraction structure determination and analysis at 1.39-2.10 A resolution |
Halalkalibacterium halodurans |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
3.2.1.156 |
D263C |
construction of a mutant engineered reducing-end xylose-releasing exooligoxylanase, that acts as a glycosynthase. Glycosynthases are engineered glycoside hydrolases, that catalyze the synthesis of glycoside from glycosyl-fluoride donors and suitable acceptors, crystal structure determination, overview |
Halalkalibacterium halodurans |
3.2.1.156 |
D263G |
construction of a mutant engineered reducing-end xylose-releasing exooligoxylanase, that acts as a glycosynthase. Glycosynthases are engineered glycoside hydrolases, that catalyze the synthesis of glycoside from glycosyl-fluoride donors and suitable acceptors, crystal structure determination, overview |
Halalkalibacterium halodurans |
3.2.1.156 |
D263N |
construction of a mutant engineered reducing-end xylose-releasing exooligoxylanase, that acts as a glycosynthase. Glycosynthases are engineered glycoside hydrolases, that catalyze the synthesis of glycoside from glycosyl-fluoride donors and suitable acceptors, crystal structure determination, overview |
Halalkalibacterium halodurans |
3.2.1.156 |
D263S |
construction of a mutant engineered reducing-end xylose-releasing exooligoxylanase, that acts as a glycosynthase. Glycosynthases are engineered glycoside hydrolases, that catalyze the synthesis of glycoside from glycosyl-fluoride donors and suitable acceptors, crystal structure determination, overview |
Halalkalibacterium halodurans |
3.2.1.156 |
Y198F |
construction of a mutant engineered reducing-end xylose-releasing exooligoxylanase, that acts as a glycosynthase. Glycosynthases are engineered glycoside hydrolases, that catalyze the synthesis of glycoside from glycosyl-fluoride donors and suitable acceptors, crystal structure determination, overview |
Halalkalibacterium halodurans |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.2.1.156 |
Halalkalibacterium halodurans |
Q9KB30 |
- |
- |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
3.2.1.156 |
additional information |
the reducing-end xylose-releasing exo-oligoxylanase is an inverting glycohydrolase, active site structure of wild-type enzyme and glycosynthase Rex mutant enzymes, overview |
Halalkalibacterium halodurans |
? |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
3.2.1.156 |
reducing-end xylose-releasing exo-oligoxylanase |
- |
Halalkalibacterium halodurans |
3.2.1.156 |
Rex |
- |
Halalkalibacterium halodurans |