EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.2.1.14 | D170A/E172Q | improved binding afinities toward substrates (GlcNAc)5, (GlcNAc)6, and GlcNbeta-4(GlcNAc)4 | Trichoderma harzianum |
3.2.1.14 | E172Q | mutant of isoform Chi42, catalytically inactive. The E172Q mutant binds chitinoligosaccharides, tetra-, penta- and hexamers, with an increasing affinity from 12 to 0.2 microM whereas no binding of chitinbiose, -triose or 3-sialyl-N-acetyllactosamine can be measured | Trichoderma harzianum |
3.2.1.14 | E317A/E172Q | affinity decrease down to 20-25% of the affinity of the E172Q reference protein for substrates (GlcNAc)5, (GlcNAc)6 | Trichoderma harzianum |
3.2.1.14 | T133D | mutation alters substrate binding specificity of Chit42 toward binding of GlcNbeta-4(GlcNAc)4 by providing a counter charge at subsite -3 | Trichoderma harzianum |
3.2.1.14 | T133D/E172Q | decrease in affinities for all ligands by about 65-80% | Trichoderma harzianum |
3.2.1.14 | T133N/E172Q | no significant loss of affinity for any of the compounds tested, increase in the selectivity for Galbeta-4(GlcNAc)4 | Trichoderma harzianum |
3.2.1.14 | W246A/E172Q | strong decrease in affinity for all ligands tested | Trichoderma harzianum |
3.2.1.14 | W48A/E172Q | strong decrease in affinity for all ligands tested | Trichoderma harzianum |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.14 | allosamidin | binding is dependent on the E172 residue, the acid/base catalyst of isoform Chit42 | Trichoderma harzianum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.14 | Trichoderma harzianum | - |
- |
- |