EC Number | Cloned (Comment) | Organism |
---|---|---|
3.4.13.19 | expression in Escherichia coli BL21 | Streptomyces coelicolor |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.4.13.19 | - |
Streptomyces coelicolor |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.4.13.19 | D22H | mutant with no residual enzyme acitvity | Streptomyces coelicolor |
3.4.13.19 | D320A | mutant with no residual enzyme acitvity | Streptomyces coelicolor |
3.4.13.19 | D320N | mutant with no residual enzyme acitvity | Streptomyces coelicolor |
3.4.13.19 | H150A | mutant with more than doubled Km-values | Streptomyces coelicolor |
3.4.13.19 | H150N | mutant with more than doubled Km-values | Streptomyces coelicolor |
3.4.13.19 | R223K | mutant with about 3fold reduced activity compared to wild-type enzyme | Streptomyces coelicolor |
3.4.13.19 | R223M | mutant with no residual enzyme acitvity | Streptomyces coelicolor |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.13.19 | citrate | - |
Streptomyces coelicolor | |
3.4.13.19 | phosphinate mimic of L-Ala-D-Asp | - |
Streptomyces coelicolor |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.13.19 | 0.14 | - |
L-Arg-D-Asp | - |
Streptomyces coelicolor | |
3.4.13.19 | 0.17 | - |
L-Tyr-D-Leu | pH 7.5, 30°C | Streptomyces coelicolor | |
3.4.13.19 | 0.37 | - |
L-Leu-D-Leu | pH 7.5, 30°C | Streptomyces coelicolor | |
3.4.13.19 | 0.55 | - |
L-Leu-D-Glu | pH 7.5, 30°C | Streptomyces coelicolor | |
3.4.13.19 | 0.56 | - |
L-Met-D-Leu | pH 7.5, 30°C | Streptomyces coelicolor | |
3.4.13.19 | 0.75 | - |
L-Leu-D-Ala | - |
Streptomyces coelicolor | |
3.4.13.19 | 0.89 | - |
L-Met-D-Glu | pH 7.5, 30°C | Streptomyces coelicolor | |
3.4.13.19 | 1.4 | - |
L-Leu-L-Leu | pH 7.5, 30°C | Streptomyces coelicolor | |
3.4.13.19 | 1.8 | - |
L-Arg-L-Asp | wild-type enzyme, pH 7.5, 30°C | Streptomyces coelicolor | |
3.4.13.19 | 2 | - |
L-Leu-L-Asp | pH 7.5, 30°C | Streptomyces coelicolor | |
3.4.13.19 | 2 | - |
L-Leu-D-Ser | pH 7.5, 30°C | Streptomyces coelicolor | |
3.4.13.19 | 2.3 | - |
L-Met-L-Leu | pH 7.5, 30°C | Streptomyces coelicolor | |
3.4.13.19 | 2.4 | - |
L-Asn-D-Glu | pH 7.5, 30°C | Streptomyces coelicolor | |
3.4.13.19 | 4.3 | - |
L-Arg-L-Asp | mutant H150A, pH 7.5, 30°C | Streptomyces coelicolor | |
3.4.13.19 | 4.4 | - |
L-Ala-D-Ala | pH 7.5, 30°C | Streptomyces coelicolor | |
3.4.13.19 | 4.9 | - |
L-Arg-L-Asp | mutant H150N, pH 7.5, 30°C | Streptomyces coelicolor | |
3.4.13.19 | 8.8 | - |
L-Arg-L-Asp | mutant R223K, pH 7.5, 30°C | Streptomyces coelicolor | |
3.4.13.19 | 9.5 | - |
L-Ala-L-Glu | pH 7.5, 30°C | Streptomyces coelicolor | |
3.4.13.19 | 13.8 | - |
L-Ala-L-Asp | pH 7.5, 30°C | Streptomyces coelicolor | |
3.4.13.19 | 16 | - |
L-Ala-L-Gln | pH 7.5, 30°C | Streptomyces coelicolor |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.13.19 | Streptomyces coelicolor | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.13.19 | ammonium sulfate precipitation, size exclusion chromatography, ion exchange chromatography and gel filtration | Streptomyces coelicolor |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.13.19 | D-Ala-D-Ala + H2O | assay at 30°C, pH 7.5 | Streptomyces coelicolor | D-Ala + D-Ala | - |
? | |
3.4.13.19 | D-Ala-L-Ala + H2O | assay at 30°C, pH 7.5 | Streptomyces coelicolor | D-Ala + L-Ala | - |
? | |
3.4.13.19 | Gly-D-Leu + H2O | assay at 30°C, pH 7.5 | Streptomyces coelicolor | Gly + D-Leu | - |
? | |
3.4.13.19 | L-Ala-D-Ala + H2O | assay at 30°C, pH 7.5 | Streptomyces coelicolor | L-Ala + D-Ala | - |
? | |
3.4.13.19 | L-Ala-L-Ala + H2O | assay at 30°C, pH 7.5 | Streptomyces coelicolor | L-Ala + L-Ala | - |
? | |
3.4.13.19 | L-Ala-L-Arg + H2O | assay at 30°C, pH 7.5 | Streptomyces coelicolor | L-Ala + L-Arg | - |
? | |
3.4.13.19 | L-Ala-L-Asp + H2O | assay at 30°C, pH 7.5 | Streptomyces coelicolor | L-Ala + L-Asp | - |
? | |
3.4.13.19 | L-Ala-L-Gln + H2O | assay at 30°C, pH 7.5 | Streptomyces coelicolor | L-Ala + L-Gln | - |
? | |
3.4.13.19 | L-Ala-L-Glu + H2O | assay at 30°C, pH 7.5 | Streptomyces coelicolor | L-Ala + L-Glu | - |
? | |
3.4.13.19 | L-Ala-L-His + H2O | assay at 30°C, pH 7.5 | Streptomyces coelicolor | L-Ala + L-His | - |
? | |
3.4.13.19 | L-Ala-L-Lys + H2O | assay at 30°C, pH 7.5 | Streptomyces coelicolor | L-Ala + L-Lys | - |
? | |
3.4.13.19 | L-Arg-D-Asp + H2O | assay at 30°C, pH 7.5 | Streptomyces coelicolor | L-Arg + D-Asp | - |
? | |
3.4.13.19 | L-Arg-Gly + H2O | assay at 30°C, pH 7.5 | Streptomyces coelicolor | L-Arg + Gly | - |
? | |
3.4.13.19 | L-Arg-L-Asp + H2O | assay at 30°C, pH 7.5 | Streptomyces coelicolor | L-Arg + L-Asp | - |
? | |
3.4.13.19 | L-Asn-D-Glu + H2O | assay at 30°C, pH 7.5 | Streptomyces coelicolor | L-Asn + D-Glu | - |
? | |
3.4.13.19 | L-Leu-D-Ala + H2O | assay at 30°C, pH 7.5 | Streptomyces coelicolor | L-Leu + D-Ala | - |
? | |
3.4.13.19 | L-Leu-D-Glu + H2O | assay at 30°C, pH 7.5 | Streptomyces coelicolor | L-Leu + D-Glu | - |
? | |
3.4.13.19 | L-Leu-D-Leu + H2O | assay at 30°C, pH 7.5 | Streptomyces coelicolor | L-Leu + D-Leu | - |
? | |
3.4.13.19 | L-Leu-D-Ser + H2O | assay at 30°C, pH 7.5 | Streptomyces coelicolor | L-Leu + D-Ser | - |
? | |
3.4.13.19 | L-Leu-L-Asp + H2O | assay at 30°C, pH 7.5 | Streptomyces coelicolor | L-Leu + L-Asp | - |
? | |
3.4.13.19 | L-Leu-L-Leu + H2O | assay at 30°C, pH 7.5 | Streptomyces coelicolor | L-Leu + L-Leu | - |
? | |
3.4.13.19 | L-Met-D-Glu + H2O | assay at 30°C, pH 7.5 | Streptomyces coelicolor | L-Met + D-Glu | - |
? | |
3.4.13.19 | L-Met-D-Leu + H2O | assay at 30°C, pH 7.5 | Streptomyces coelicolor | L-Met + D-Leu | - |
? | |
3.4.13.19 | L-Met-L-Leu + H2O | assay at 30°C, pH 7.5 | Streptomyces coelicolor | L-Met + L-Leu | - |
? | |
3.4.13.19 | L-Tyr-D-Leu + H2O | assay at 30°C, pH 7.5 | Streptomyces coelicolor | L-Tyr + D-Leu | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.4.13.19 | renal dipeptidase | - |
Streptomyces coelicolor |
3.4.13.19 | Sco3058 | - |
Streptomyces coelicolor |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.13.19 | 30 | - |
assay at | Streptomyces coelicolor |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.13.19 | additional information | - |
L-Arg-L-Asp | mutant D22H, value below 0.03, pH 7.5, 30°C | Streptomyces coelicolor | |
3.4.13.19 | additional information | - |
L-Arg-L-Asp | mutant D320A, value below 0.03, pH 7.5, 30°C | Streptomyces coelicolor | |
3.4.13.19 | additional information | - |
L-Arg-L-Asp | mutant D320N, value below 0.03, pH 7.5, 30°C | Streptomyces coelicolor | |
3.4.13.19 | additional information | - |
L-Arg-L-Asp | mutant R223M, value below 0.03, pH 7.5, 30°C | Streptomyces coelicolor | |
3.4.13.19 | 0.4 | - |
L-Arg-L-Asp | mutant R223K, pH 7.5, 30°C | Streptomyces coelicolor | |
3.4.13.19 | 0.7 | - |
L-Arg-L-Asp | mutant H150N, pH 7.5, 30°C | Streptomyces coelicolor | |
3.4.13.19 | 7.2 | - |
L-Arg-L-Asp | mutant H150A, pH 7.5, 30°C | Streptomyces coelicolor | |
3.4.13.19 | 21 | - |
L-Leu-D-Leu | pH 7.5, 30°C | Streptomyces coelicolor | |
3.4.13.19 | 24 | - |
L-Tyr-D-Leu | pH 7.5, 30°C | Streptomyces coelicolor | |
3.4.13.19 | 107 | - |
L-Arg-D-Asp | - |
Streptomyces coelicolor | |
3.4.13.19 | 132 | - |
L-Arg-L-Asp | wild-type enzyme, pH 7.5, 30°C | Streptomyces coelicolor | |
3.4.13.19 | 135 | - |
L-Leu-D-Glu | pH 7.5, 30°C | Streptomyces coelicolor | |
3.4.13.19 | 140 | - |
L-Leu-L-Leu | pH 7.5, 30°C | Streptomyces coelicolor | |
3.4.13.19 | 140 | - |
L-Ala-L-Gln | pH 7.5, 30°C | Streptomyces coelicolor | |
3.4.13.19 | 160 | - |
L-Leu-D-Ala | - |
Streptomyces coelicolor | |
3.4.13.19 | 160 | - |
L-Leu-L-Asp | pH 7.5, 30°C | Streptomyces coelicolor | |
3.4.13.19 | 194 | - |
L-Met-D-Leu | pH 7.5, 30°C | Streptomyces coelicolor | |
3.4.13.19 | 220 | - |
L-Ala-L-Glu | pH 7.5, 30°C | Streptomyces coelicolor | |
3.4.13.19 | 288 | - |
L-Asn-D-Glu | pH 7.5, 30°C | Streptomyces coelicolor | |
3.4.13.19 | 310 | - |
L-Met-L-Leu | pH 7.5, 30°C | Streptomyces coelicolor | |
3.4.13.19 | 340 | - |
L-Met-D-Glu | pH 7.5, 30°C | Streptomyces coelicolor | |
3.4.13.19 | 400 | - |
L-Leu-D-Ser | pH 7.5, 30°C | Streptomyces coelicolor | |
3.4.13.19 | 750 | - |
L-Ala-D-Ala | pH 7.5, 30°C | Streptomyces coelicolor | |
3.4.13.19 | 1400 | - |
L-Ala-L-Asp | pH 7.5, 30°C | Streptomyces coelicolor |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.4.13.19 | 7.5 | - |
assay at | Streptomyces coelicolor |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.4.13.19 | metabolism | glutathione metabolism | Streptomyces coelicolor |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.13.19 | 0.0022 | - |
D-Ala-L-Ala | pH 7.5, 30°C | Streptomyces coelicolor | |
3.4.13.19 | 0.046 | - |
L-Arg-L-Asp | mutant R223K, pH 7.5, 30°C | Streptomyces coelicolor | |
3.4.13.19 | 0.072 | - |
L-Ala-L-Lys | pH 7.5, 30°C | Streptomyces coelicolor | |
3.4.13.19 | 0.13 | - |
D-Ala-D-Ala | pH 7.5, 30°C | Streptomyces coelicolor | |
3.4.13.19 | 0.14 | - |
L-Arg-L-Asp | mutant H150N, pH 7.5, 30°C | Streptomyces coelicolor | |
3.4.13.19 | 0.18 | - |
L-Ala-L-Arg | pH 7.5, 30°C | Streptomyces coelicolor | |
3.4.13.19 | 0.45 | - |
L-Ala-L-His | pH 7.5, 30°C | Streptomyces coelicolor | |
3.4.13.19 | 1.7 | - |
L-Arg-L-Asp | mutant H150A, pH 7.5, 30°C | Streptomyces coelicolor | |
3.4.13.19 | 8.4 | - |
L-Ala-L-Gln | pH 7.5, 30°C | Streptomyces coelicolor | |
3.4.13.19 | 14 | - |
L-Ala-L-Ala | pH 7.5, 30°C | Streptomyces coelicolor | |
3.4.13.19 | 22 | - |
L-Arg-Gly | pH 7.5, 30°C | Streptomyces coelicolor | |
3.4.13.19 | 24 | - |
L-Ala-L-Glu | pH 7.5, 30°C | Streptomyces coelicolor | |
3.4.13.19 | 33 | - |
Gly-D-Leu | pH 7.5, 30°C | Streptomyces coelicolor | |
3.4.13.19 | 56 | - |
L-Leu-D-Leu | pH 7.5, 30°C | Streptomyces coelicolor | |
3.4.13.19 | 72 | - |
L-Arg-L-Asp | wild-type enzyme, pH 7.5, 30°C | Streptomyces coelicolor | |
3.4.13.19 | 83 | - |
L-Leu-L-Asp | pH 7.5, 30°C | Streptomyces coelicolor | |
3.4.13.19 | 100 | - |
L-Leu-L-Leu | pH 7.5, 30°C | Streptomyces coelicolor | |
3.4.13.19 | 100 | - |
L-Ala-L-Asp | pH 7.5, 30°C | Streptomyces coelicolor | |
3.4.13.19 | 120 | - |
L-Asn-D-Glu | pH 7.5, 30°C | Streptomyces coelicolor | |
3.4.13.19 | 140 | - |
L-Met-L-Leu | pH 7.5, 30°C | Streptomyces coelicolor | |
3.4.13.19 | 150 | - |
L-Tyr-D-Leu | pH 7.5, 30°C | Streptomyces coelicolor | |
3.4.13.19 | 170 | - |
L-Ala-D-Ala | pH 7.5, 30°C | Streptomyces coelicolor | |
3.4.13.19 | 200 | - |
L-Leu-D-Ser | pH 7.5, 30°C | Streptomyces coelicolor | |
3.4.13.19 | 210 | - |
L-Leu-D-Ala | pH 7.5, 30°C | Streptomyces coelicolor | |
3.4.13.19 | 250 | - |
L-Leu-D-Glu | pH 7.5, 30°C | Streptomyces coelicolor | |
3.4.13.19 | 350 | - |
L-Met-D-Leu | pH 7.5, 30°C | Streptomyces coelicolor | |
3.4.13.19 | 380 | - |
L-Met-D-Glu | pH 7.5, 30°C | Streptomyces coelicolor | |
3.4.13.19 | 760 | - |
L-Arg-D-Asp | pH 7.5, 30°C | Streptomyces coelicolor |