EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.1.1.73 | additional information | ferulic acid release by recombinant FaeB or FaeC is increased considerably by 17.6fold and 16fold, respectively in the presence of Thermomyces lanuginosus xylanase | Aspergillus oryzae |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.1.73 | PCR product cloned into the pGEM-T Easy vector. FaeB and FaeC fragments subcloned into EcoRI-XbaI-digested pPICZalphaA expression vector. FaeB and FaeC expressed in Pichia pastoris GS115 by fusing to the Saccharomyces cerevisiae alpha-factor secretion signal peptide | Aspergillus oryzae |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.1.73 | 55000 | - |
x * 61000, purified recombinant FaeB, SDS-PAGE. x * 75000, purified recombinant FaeC, SDS-PAGE. x * 55000, purified recombinant FaeB and FaeC after N-deglycosylation with endo-H, SDS-PAGE | Aspergillus oryzae |
3.1.1.73 | 61000 | - |
x * 61000, purified recombinant FaeB, SDS-PAGE. x * 75000, purified recombinant FaeC, SDS-PAGE. x * 55000, purified recombinant FaeB and FaeC after N-deglycosylation with endo-H, SDS-PAGE | Aspergillus oryzae |
3.1.1.73 | 75000 | - |
x * 61000, purified recombinant FaeB, SDS-PAGE. x * 75000, purified recombinant FaeC, SDS-PAGE. x * 55000, purified recombinant FaeB and FaeC after N-deglycosylation with endo-H, SDS-PAGE | Aspergillus oryzae |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.1.73 | Aspergillus oryzae | - |
- |
- |
3.1.1.73 | Aspergillus oryzae RIB 40 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.1.73 | FaeB and FaeC, in a two-step procedure using anion-exchange chromatography and gel filtration | Aspergillus oryzae |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.1.73 | ? | x * 61000, purified recombinant FaeB, SDS-PAGE. x * 75000, purified recombinant FaeC, SDS-PAGE. x * 55000, purified recombinant FaeB and FaeC after N-deglycosylation with endo-H, SDS-PAGE | Aspergillus oryzae |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.1.73 | FAEB | - |
Aspergillus oryzae |
3.1.1.73 | FaeC | - |
Aspergillus oryzae |
3.1.1.73 | feruloyl esterase | - |
Aspergillus oryzae |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.73 | 55 | - |
purified recombinant FaeB and FaeC retain more than 90% of their activity after incubation at 55°C for 30 min. FaeC retains 40% of its activity after treatment at 65°C, whereas FaeB almost loses its activity | Aspergillus oryzae |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.73 | 6 | - |
purified recombinant FaeB and FaeC | Aspergillus oryzae |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.73 | 6 | 7 | purified recombinant FaeB and FaeC show more than 90% of their maximum activity | Aspergillus oryzae |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.73 | 3 | 9 | purified recombinant FaeB | Aspergillus oryzae |
3.1.1.73 | 7 | 10 | purified recombinant FaeC | Aspergillus oryzae |