Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Jin, L.; Liu, Z.; Zheng, Y.; Shen, Y.
    Identification and characterization of Serratia marcescens ZJB-09104, a nitrile-converting bacterium (2010), World J. Microbiol. Biotechnol., 26, 817-823.
    View publication on PubMed

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.84 Cobalt belongs to the cobalt NHase family of enzymes Serratia marcescens

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.84 Serratia marcescens
-
-
-
4.2.1.84 Serratia marcescens ZJB-09104
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.84 2-cyanopyridine + H2O
-
 Serratia marcescens pyridine-2-carbamide
-
 ?
4.2.1.84 2-cyanopyridine + H2O
-
 Serratia marcescens ZJB-09104 pyridine-2-carbamide
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.84 NHase
-
 Serratia marcescens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.84 50
-
-
 Serratia marcescens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.1.84 50 65 the enzyme activity gradually reduces with the further increase of temperature from 50 to 65°C, the thermostability of NHase is comparatively good under 50°C, while the enzyme activity is obviously lost when the cells are preincubated over 50°C Serratia marcescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.84 8
-
 the optimum nitrile hydratase activity is observed in the Tris-HCl buffer at a pH of 8.0 Serratia marcescens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.2.1.84 4.3 10.4 at pH value of 4.3 and 10.4 enzyme activities are reduced to 24 and 25% of the maximum activity, respectively Serratia marcescens