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Literature summary extracted from

  • Ma, S.M.; Li, J.W.; Choi, J.W.; Zhou, H.; Lee, K.K.; Moorthie, V.A.; Xie, X.; Kealey, J.T.; Da Silva, N.A.; Vederas, J.C.; Tang, Y.
    Complete reconstitution of a highly reducing iterative polyketide synthase (2009), Science, 326, 589-592.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.161 expressed in Saccharomyces cerevisiae Aspergillus terreus

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.161 Aspergillus terreus
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-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.161
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 Aspergillus terreus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.161 acetyl-CoA + 8 malonyl-CoA + 11 NADPH + S-adenosyl-L-methionine + 11 H+ accessory protein LovC is needed: Loading of the megasynthase by malonyl-CoA is presumably followed by decarboxylation to yield the acetyl starter unit. Each round of Claisen condensation is catalyzed by the KS domain, whereas the growing polyketide is tethered to the phosphopantetheinyl arm of the ACP. After each condensation, the polyketide is subjected to a different combination of tailoring, which can include alpha-methylation by the MT domain, beta-ketoreduction by the KR domain, beta-dehydration by the DH domain, and alpha-beta-enoylreduction by the dissociated LovC. The different tailoring permutations after each round of chain extension yield a triene-containing hexaketide thioester that can undergo a stereospecific Diels-Alder cyclization to yield the decalin portion. After formation of the nonaketide, the chain is released to yield the ring-open form. In the absence of LovC, methylated hexa- and heptaketide pyrones are the primary products. Aspergillus terreus dihydromonacolin L + 9 CoA + 8 CO2 + 11 NADP+ + S-adenosyl-L-homocysteine + 6 H2O
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 ir

Synonyms

EC Number Synonyms Comment Organism
2.3.1.161 lovastatin nonaketide synthase
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 Aspergillus terreus
2.3.1.161 LovB
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 Aspergillus terreus

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.161 NADPH
-
 Aspergillus terreus
2.3.1.161 S-adenosyl-L-methionine
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 Aspergillus terreus