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Literature summary extracted from
- Differential roles of archaeal box H/ACA proteins in guide RNA-dependent and independent pseudouridine formation (2007), RNA Biol., 4, 101-109.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.4.99.25 | aGar1 protein | the aCbf5 alone can produce pseudouridine55 in tRNAs that contain the canonical 3-CCA sequence. This activity is stimulated by aGar1 | Methanocaldococcus jannaschii | |
| 5.4.99.25 | aNop10 protein | aCbf5 and aGar1 together can function as a tRNA pseudouridine55 synthase in a guide RNA-independent manner. This activity is enhanced by aNop10, but not by L7Ae | Methanocaldococcus jannaschii | |
| 5.4.99.25 | protein aGar1 | aCbf5 alone can also produce Y55 in tRNAs that contain the canonical 3'-CCA sequence and this activity is stimulated by aGar1 | Methanocaldococcus jannaschii | |
| 5.4.99.25 | protein aNOP10 | aCbf5 and aGar1 together can function as a tRNA Y55 synthase in a guide RNA-independent manner. This activity is enhanced by aNop10, but not by L7Ae | Methanocaldococcus jannaschii |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.4.99.25 | - |
Methanocaldococcus jannaschii |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.4.99.25 | Methanocaldococcus jannaschii | - |
- |
- |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 5.4.99.25 | tRNA uridine55 = tRNA pseudouridine55 | the mechanism of uridine55 recognition by Cbf5 and the bacterial TruB are different | Methanocaldococcus jannaschii |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.99.25 | tRNA uridine55 | aCbf5 and aGar1 together can function as a tRNA pseudouridine55 synthase in a guide RNA-independent manner. This activity is enhanced by aNop10, but not by L7Ae. The aCbf5 alone can also produce pseudouridine55 in tRNAs that contain the canonical 3-CCA sequence and this activity is stimulated by aGar1. The presence of C (or U) and A at tRNA position 56 and 58, respectively are not essential for Cbf5-mediated PSI55 formation. Variation in the structure of the anticodon arm of the tRNA does not affect the PSI55 synthase activity | Methanocaldococcus jannaschii | tRNA pseudouridine55 | - |
? |  |
| 5.4.99.25 | tRNATrp uridine55 | tRNATrp containing or lacking 3'-CCA. aCbf5 and aGar1 together can function as a tRNA Psi55 synthase in a guide RNA-independent manner. This activity is enhanced by aNop10, but not by L7Ae. The aCbf5 alone can also produce Psi55 in tRNAs that contain the canonical 3'-CCA sequence and this activity is stimulated by aGar1. tRNAs lacking 3'-CCA can be modified only by the aCbf5-aGar1 complex. The presence of conserved C (or U) and A at tRNA positions 56 and 58, respectively, is not essential for aCbf5-mediated Psi55 formation | Methanocaldococcus jannaschii | tRNATrp pseudouridine55 | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.25 | 68 | - |
assay at | Methanocaldococcus jannaschii |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.25 | 7 | - |
assay at | Methanocaldococcus jannaschii |
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Release 2023.1 (January 2023)
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