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Literature summary extracted from

  • Tsai, S.C.; Miercke, L.J.W.; Krucinski, J.; Gokhale, R.; Chen, J.C.H.; Foster, P.G.; Cane, D.E.; Khosla, C.; Stroud, R.M.
    Crystal structure of the macrocycle-forming thioesterase domain of the erythromycin polyketide synthase: Versatility from a unique substrate channel (2001), Proc. Natl. Acad. Sci. USA, 98, 14808-14813.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.3.1.94 biotechnology polyketide synthases synthesize the polyketide cores of biologically active compounds, including natural products that have become important pharmaceuticals, like erythromycin Saccharopolyspora erythraea

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.94 the thioesterase domain from deoxyerythronolide B synthase is cloned for expression in Escherichia coli cells Saccharopolyspora erythraea

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.1.94 the crystal structure of the thioesterase domain from deoxyerythronolide B synthase is solved to 2.8 A resolution Saccharopolyspora erythraea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.94 33000
-
 thioesterase domain, 2 * 33000 Saccharopolyspora erythraea
2.3.1.94 66000
-
 thioesterase domain, homodimer Saccharopolyspora erythraea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.94 propanoyl-CoA + 6 (2S)-methylmalonyl-CoA + 6 NADPH + 6 H+ Saccharopolyspora erythraea
-
 6-deoxyerythronolide B + 7 CoA + 6 CO2 + H2O + 6 NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.94 Saccharopolyspora erythraea Q03133
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.94 using a Ni-agarose and a Poros HQ column Saccharopolyspora erythraea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.94 propanoyl-CoA + 6 (2S)-methylmalonyl-CoA + 6 NADPH + 6 H+
-
 Saccharopolyspora erythraea 6-deoxyerythronolide B + 7 CoA + 6 CO2 + H2O + 6 NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.94 homodimer thioesterase domain, 2 * 33000 Saccharopolyspora erythraea
2.3.1.94 More deoxyerythronolide B synthase consists of several modules, formed by acyltransferases, acyl carrier proteins, ketosynthases, ketoreductases, dehydratases, enoylreductases and thioesterases Saccharopolyspora erythraea

Synonyms

EC Number Synonyms Comment Organism
2.3.1.94 DEBS
-
 Saccharopolyspora erythraea
2.3.1.94 deoxyerythronolide B synthase
-
 Saccharopolyspora erythraea
2.3.1.94 erythromycin polyketide synthase
-
 Saccharopolyspora erythraea
2.3.1.94 PKS
-
 Saccharopolyspora erythraea
2.3.1.94 polyketide synthase
-
 Saccharopolyspora erythraea