EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.4.1 | expressed in Escherichia coli strains BL21 (DE3) and B834 (DE3) | Pseudomonas aeruginosa |
2.7.4.1 | expressed in Escherichia coli strains BL21 (DE3) and B834 (DE3) | Sinorhizobium meliloti |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.7.4.1 | hanging drop vapor diffusion method, using 0.1 M Bis-Tris (pH 7.5), 0.1 M Na-formate, 0.1 M Li-sulfate, and 0.3 M NDSB 211 | Sinorhizobium meliloti |
2.7.4.1 | hanging drop vapor diffusion method, using 0.1 M HEPES-K (pH 7.0), 5% tacsimate, and 10% PEG MME 5K | Pseudomonas aeruginosa |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.7.4.1 | D148A | the mutant shows strongly reduced activity compared to the wild type enzyme | Sinorhizobium meliloti |
2.7.4.1 | D223A | the mutant shows strongly reduced activity compared to the wild type enzyme | Sinorhizobium meliloti |
2.7.4.1 | D307A | the mutant shows strongly reduced activity compared to the wild type enzyme | Pseudomonas aeruginosa |
2.7.4.1 | D320A | the mutant shows reduced activity compared to the wild type enzyme | Pseudomonas aeruginosa |
2.7.4.1 | D323A | the mutant shows reduced activity compared to the wild type enzyme | Pseudomonas aeruginosa |
2.7.4.1 | D362A | the mutant shows strongly reduced activity compared to the wild type enzyme | Pseudomonas aeruginosa |
2.7.4.1 | D437A | the mutant shows strongly reduced activity compared to the wild type enzyme | Pseudomonas aeruginosa |
2.7.4.1 | D93A | the mutant shows strongly reduced activity compared to the wild type enzyme | Sinorhizobium meliloti |
2.7.4.1 | E304A | the mutant shows strongly reduced activity compared to the wild type enzyme | Pseudomonas aeruginosa |
2.7.4.1 | E90A | the mutant shows strongly reduced activity compared to the wild type enzyme | Sinorhizobium meliloti |
2.7.4.1 | K311A | the mutant shows strongly reduced activity compared to the wild type enzyme | Pseudomonas aeruginosa |
2.7.4.1 | K473A | the mutant shows reduced activity compared to the wild type enzyme | Pseudomonas aeruginosa |
2.7.4.1 | K97A | the mutant shows strongly reduced activity compared to the wild type enzyme | Sinorhizobium meliloti |
2.7.4.1 | Q202A | the mutant shows strongly reduced activity compared to the wild type enzyme | Sinorhizobium meliloti |
2.7.4.1 | Q416A | the mutant shows strongly reduced activity compared to the wild type enzyme | Pseudomonas aeruginosa |
2.7.4.1 | R149A | the mutant shows strongly reduced activity compared to the wild type enzyme | Sinorhizobium meliloti |
2.7.4.1 | R205A | the mutant shows strongly reduced activity compared to the wild type enzyme | Sinorhizobium meliloti |
2.7.4.1 | R209A | the mutant shows strongly reduced activity compared to the wild type enzyme | Sinorhizobium meliloti |
2.7.4.1 | R263A | the mutant shows almost wild type activity | Sinorhizobium meliloti |
2.7.4.1 | R316A | the mutant shows reduced activity compared to the wild type enzyme | Pseudomonas aeruginosa |
2.7.4.1 | R325A | the mutant shows reduced activity compared to the wild type enzyme | Pseudomonas aeruginosa |
2.7.4.1 | R363A | the mutant shows almost no activity compared to the wild type enzyme | Pseudomonas aeruginosa |
2.7.4.1 | R419A | the mutant shows strongly reduced activity compared to the wild type enzyme | Pseudomonas aeruginosa |
2.7.4.1 | R423A | the mutant shows strongly reduced activity compared to the wild type enzyme | Pseudomonas aeruginosa |
2.7.4.1 | R477A | the mutant shows increased activity compared to the wild type enzyme | Pseudomonas aeruginosa |
2.7.4.1 | W194A | the mutant shows strongly reduced activity compared to the wild type enzyme | Sinorhizobium meliloti |
2.7.4.1 | W408A | the mutant shows strongly reduced activity compared to the wild type enzyme | Pseudomonas aeruginosa |
2.7.4.1 | Y233A | the mutant shows strongly reduced activity compared to the wild type enzyme | Sinorhizobium meliloti |
2.7.4.1 | Y447A | the mutant shows strongly reduced activity compared to the wild type enzyme | Pseudomonas aeruginosa |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.4.1 | Mg2+ | no activity in the absence of a divalent metal cation, Mg2+ is the most effective metal, whereas low activity is observed with Co2+ or Ni2+ and no activity is observed in the presence of Mn2+ or Ca2+ | Pseudomonas aeruginosa | |
2.7.4.1 | Mg2+ | no activity in the absence of a divalent metal cation, Mg2+ is the most effective metal, whereas low activity is observed with Co2+ or Ni2+ and no activity is observed in the presence of Mn2+ or Ca2+ | Sinorhizobium meliloti |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.4.1 | 97000 | - |
gel filtration | Pseudomonas aeruginosa |
2.7.4.1 | 124500 | - |
gel filtration | Sinorhizobium meliloti |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.4.1 | Pseudomonas aeruginosa | - |
- |
- |
2.7.4.1 | Sinorhizobium meliloti | Q92SA6 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.4.1 | - |
Pseudomonas aeruginosa |
2.7.4.1 | - |
Sinorhizobium meliloti |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.4.1 | ATP + (phosphate)n | - |
Pseudomonas aeruginosa | ADP + (phosphate)n+1 | - |
? | |
2.7.4.1 | ATP + (phosphate)n | - |
Sinorhizobium meliloti | ADP + (phosphate)n+1 | - |
? | |
2.7.4.1 | additional information | PA3455 protein is also active with (phosphate)3 as a phosphodonor, but its activity and substrate affinity are lower than those with (phosphate)12-13 | Pseudomonas aeruginosa | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.4.1 | homodimer | x-ray crystallography | Pseudomonas aeruginosa |
2.7.4.1 | homotetramer | x-ray crystallography | Sinorhizobium meliloti |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.4.1 | family-2 polyphosphate kinase | - |
Pseudomonas aeruginosa |
2.7.4.1 | family-2 polyphosphate kinase | - |
Sinorhizobium meliloti |
2.7.4.1 | PA3455 protein | - |
Pseudomonas aeruginosa |
2.7.4.1 | PPK2 | - |
Pseudomonas aeruginosa |
2.7.4.1 | PPK2 | - |
Sinorhizobium meliloti |
2.7.4.1 | SMc02148 protein | - |
Sinorhizobium meliloti |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.4.1 | 8 | 9.5 | - |
Pseudomonas aeruginosa |
2.7.4.1 | 8 | 9.5 | - |
Sinorhizobium meliloti |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.7.4.1 | ATP | - |
Pseudomonas aeruginosa | |
2.7.4.1 | ATP | - |
Sinorhizobium meliloti |