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Literature summary extracted from

  • Okazaki, M.; Higuchi, K.; Hanawa, Y.; Shiraiwa, Y.; Ezura, H.
    Cloning and characterization of a Chlamydomonas reinhardtii cDNA arylalkylamine N-acetyltransferase and its use in the genetic engineering of melatonin content in the Micro-Tom tomato (2009), J. Pineal Res., 46, 373-382.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.87 AANAT, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic tree, expression in Micro-Tom, a model cultivar of Solanum lycopersicum, transgenic Micro-Tom exhibits higher melatonin content compared with wild type. Expression of N-terminally GST-tagged AANAT in Escherichia coli strain BL21 Chlamydomonas reinhardtii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.87 acetyl-CoA + serotonin Chlamydomonas reinhardtii rate-limiting in melatonin, i.e. N-acetyl-5-methoxytryptamine synthesis CoA + N-acetylserotonin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.87 Chlamydomonas reinhardtii
-
 cDNA-coded arylalkylamine N-acetyltransferase
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.3.1.87 additional information lacks cAMP-dependent protein kinase phosphorylation sites in the N- and C-terminal regions Chlamydomonas reinhardtii

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.87 recombinant N-terminally GST-tagged AANAT from Escherichia coli strain BL21 by glutathione affinity chromatography Chlamydomonas reinhardtii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.87 acetyl-CoA + serotonin
-
 Chlamydomonas reinhardtii CoA + N-acetylserotonin
-
 ?
2.3.1.87 acetyl-CoA + serotonin rate-limiting in melatonin, i.e. N-acetyl-5-methoxytryptamine synthesis Chlamydomonas reinhardtii CoA + N-acetylserotonin
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.87 AANAT
-
 Chlamydomonas reinhardtii
2.3.1.87 arylalkylamine N-acetyltransferase
-
 Chlamydomonas reinhardtii

General Information

EC Number General Information Comment Organism
2.3.1.87 metabolism the enzyme catalyzes the rate-limiting step in melatonin synthesis Chlamydomonas reinhardtii