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Literature summary extracted from

  • Hoffmann, M.; Seidel, J.; Einsle, O.
    CcpA from Geobacter sulfurreducens is a basic di-heme cytochrome c peroxidase (2009), J. Mol. Biol., 393, 951-965.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.5 expression in Escherichia coli Geobacter sulfurreducens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.11.1.5 sitting-drop vapor diffusion, 16% PEG 10000, 0.1 HEPES/NaOH, pH 7.4, 293K, wild-type enzyme, space group P1, 2.00 A resolution, mutant enzyme G94K/K97Q/R100I, space group P4321, 3.21 A resolution, mutant enzyme S134P/V135K, space group P21, 2.40 A resolution, mutant enzame S134P, space group P21, 2.40 A resolution Geobacter sulfurreducens

Protein Variants

EC Number Protein Variants Comment Organism
1.11.1.5 A124K/K128A site-directed mutagenesis, no significant changes Geobacter sulfurreducens
1.11.1.5 G94K/K97Q/R100I site-directed mutagenesis, triple point mutant is created to mimic the critical loop region of, but its crystal structure reveals that the inactive, bishistidinyl-coordinated form of the active-site heme group is retained Geobacter sulfurreducens
1.11.1.5 S134P site-directed mutagenesis, distortion of the loop region, accompanied by an opening of the active-site loop, leaving the enzyme in a constitutively active state Geobacter sulfurreducens
1.11.1.5 S134P/V135K site-directed mutagenesis, distortion of the loop region, accompanied by an opening of the active-site loop, leaving the enzyme in a constitutively active state Geobacter sulfurreducens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.5 0.0062
-
 H2O2 pH5.5 Geobacter sulfurreducens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.11.1.5 2 ferrocytochrome c + H2O2 Geobacter sulfurreducens
-
 2 ferricytochrome c + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.5 Geobacter sulfurreducens Q749D0 DSM 12127
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.5 chromatography Geobacter sulfurreducens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.11.1.5 26.4
-
 2,2’-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) and H2O2 as electron donor and acceptor Geobacter sulfurreducens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.5 2 ferrocytochrome c + H2O2
-
 Geobacter sulfurreducens 2 ferricytochrome c + H2O
-
 ?
1.11.1.5 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2
-
 Geobacter sulfurreducens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.5 dimer
-
 Geobacter sulfurreducens

Synonyms

EC Number Synonyms Comment Organism
1.11.1.5 CcpA bacterial di-heme cytochrome c peroxidase Geobacter sulfurreducens
1.11.1.5 cytochrome c peroxidase
-
 Geobacter sulfurreducens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.11.1.5 15.5
-
 H2O2 pH 5.5 Geobacter sulfurreducens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.5 5.5
-
-
 Geobacter sulfurreducens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.11.1.5 5 8
-
 Geobacter sulfurreducens

General Information

EC Number General Information Comment Organism
1.11.1.5 physiological function bacterial di-heme cytochrome c peroxidases (CcpAs) protect the cell from reactive oxygen species by reducing hydrogen peroxide to water Geobacter sulfurreducens