EC Number | Cloned (Comment) | Organism |
---|---|---|
4.2.1.22 | expressed as a GST-fusion protein in Escherichia coli | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.2.1.22 | H67A | mutant is comparable to wild-type, specific activity and Km values for L-Ser, L-homocysteine comparable to wild-type | Homo sapiens |
4.2.1.22 | R266G | patient mutation , mutant protein shows instability and extensive degradation during thrombin treatment. A GST-R266G fusion protein does not exhibit any detectable activity unlike the GST-tagged wild-type CBS | Homo sapiens |
4.2.1.22 | R266K | mutant is moderately pyridoxal 5'-phosphate responsive, Km value for serine is slightly elevated compared to wild-type CBS, Km value for homocysteine slightly lower compared to wild-type | Homo sapiens |
4.2.1.22 | R266M | R266M mutant shows a significantly lower basal activity, Km value for serine is slightly elevated compared to wild-type CBS, Km value for homocysteine slightly lower compared to wild-type, R266M mutant shows dramatic differences in the ferrous state. The electrostatic interaction between C52 and R266 is critical for stabilizing the ferrous heme and its disruption leads to the facile formation of a 424 nm (C-424) absorbing ferrous species, which is inactive, compared to the active 449 nm ferrous species for wild-type CBS. Resonance Raman studies on the R266M mutant reveal that the kinetics of C52 rebinding after Fe-CO photolysis are comparable to that of wild-type CBS | Homo sapiens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.2.1.22 | CO | - |
Homo sapiens | |
4.2.1.22 | HgCl2 | - |
Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.1.22 | 1.3 | - |
L-homocysteine | mutant R266K | Homo sapiens | |
4.2.1.22 | 1.6 | - |
L-homocysteine | mutant R266K | Homo sapiens | |
4.2.1.22 | 2 | - |
L-cysteine | wild-type | Homo sapiens | |
4.2.1.22 | 3.4 | - |
L-homocysteine | mutant H67A | Homo sapiens | |
4.2.1.22 | 4.4 | - |
L-cysteine | mutant R266K | Homo sapiens | |
4.2.1.22 | 5 | - |
L-homocysteine | wild-type | Homo sapiens | |
4.2.1.22 | 5.4 | - |
L-cysteine | mutant H67A | Homo sapiens | |
4.2.1.22 | 5.5 | - |
L-cysteine | mutant R266K | Homo sapiens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.1.22 | Homo sapiens | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.2.1.22 | using a glutathione sepharose column | Homo sapiens |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
4.2.1.22 | 0.68 | - |
without pyridoxal 5'-phosphate, without S-adenosyl-L-methionine, mutant R266M | Homo sapiens |
4.2.1.22 | 1.91 | - |
without pyridoxal 5'-phosphate, without S-adenosyl-L-methionine, mutant R266K | Homo sapiens |
4.2.1.22 | 2.31 | - |
without pyridoxal 5'-phosphate, without S-adenosyl-L-methionine, mutant H67A | Homo sapiens |
4.2.1.22 | 3.6 | - |
without pyridoxal 5'-phosphate, without S-adenosyl-L-methionine, wild-type | Homo sapiens |
4.2.1.22 | 3.9 | - |
with pyridoxal 5'-phosphate, with S-adenosyl-L-methionine, mutant R266M | Homo sapiens |
4.2.1.22 | 5.2 | - |
with pyridoxal 5'-phosphate, with S-adenosyl-L-methionine, mutant H67A | Homo sapiens |
4.2.1.22 | 5.2 | - |
with pyridoxal 5'-phosphate, with S-adenosyl-L-methionine, wild-type | Homo sapiens |
4.2.1.22 | 5.71 | - |
with pyridoxal 5'-phosphate, with S-adenosyl-L-methionine, mutant R266K | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.22 | L-cysteine + L-homocysteine | - |
Homo sapiens | L-cystathionine + H2S | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.2.1.22 | dimer | - |
Homo sapiens |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.2.1.22 | CBS | - |
Homo sapiens |
4.2.1.22 | cystathionine beta-synthase | - |
Homo sapiens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.1.22 | 37 | - |
assay at | Homo sapiens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.2.1.22 | 8.6 | - |
assay at | Homo sapiens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
4.2.1.22 | heme | - |
Homo sapiens | |
4.2.1.22 | pyridoxal 5'-phosphate | - |
Homo sapiens | |
4.2.1.22 | S-adenosyl-L-methionine | - |
Homo sapiens |