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Literature summary extracted from
- Antimicrobial effect by extracts of rhizome of Alpinia officinarum Hance may relate to its inhibition of beta-ketoacyl-ACP reductase (2008), J. Enzyme Inhib. Med. Chem., 23, 362-368.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 1.1.1.100 | drug development | galangal extract inhibits FabG, thereby displaying antibacterial ability | Staphylococcus aureus |
| 1.1.1.100 | drug development | galangal extract inhibits FabG, thereby displaying antibacterial ability | Pseudomonas aeruginosa |
| 1.1.1.100 | drug development | galangal extract inhibits FabG, thereby displaying antibacterial ability | Streptococcus pneumoniae |
| 1.1.1.100 | drug development | galangal extract inhibits FabG, thereby displaying antibacterial ability | Streptococcus sp. |
| 1.1.1.100 | drug development | galangal extract inhibits FabG, thereby displaying antibacterial ability | synthetic construct |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.1.1.100 | overexpressed in Escherichia coli BL21 (DE3) | synthetic construct |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.100 | kaempferol | - |
synthetic construct |  |
| 1.1.1.100 | additional information | FabG is inhibited by extract of galangal (rhizome of Alpinia officinarum). Minimum inhibitory concentration is above 1.28 mM | Pseudomonas aeruginosa | |
| 1.1.1.100 | additional information | FabG is inhibited by extract of galangal (rhizome of Alpinia officinarum). Minimum inhibitory concentration is 0.32-0.64 mM | Staphylococcus aureus | |
| 1.1.1.100 | additional information | FabG is inhibited by extract of galangal (rhizome of Alpinia officinarum). Minimum inhibitory concentration is 0.01-0.02 mM | Streptococcus pneumoniae | |
| 1.1.1.100 | additional information | FabG is inhibited by extract of galangal (rhizome of Alpinia officinarum). Minimum inhibitory concentration is 0.025-0.05 mM | Streptococcus sp. | |
| 1.1.1.100 | additional information | FabG is inhibited by extract of galangal (rhizome of Alpinia officinarum), strongest inhibition with 40% ethanol extract of galangal. Inhibition is consisted of both reversible and irreversible inhibition. Inhibits FabG in a competitive pattern against NADPH, irreversible inhibition presents two phases (slow and fast one) | synthetic construct | |
| 1.1.1.100 | quercetin | - |
synthetic construct | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.100 | Pseudomonas aeruginosa | - |
ATCC27853 | - |
| 1.1.1.100 | Pseudomonas aeruginosa ATCC 27853 | - |
ATCC27853 | - |
| 1.1.1.100 | Staphylococcus aureus | - |
ATCC25923 | - |
| 1.1.1.100 | Staphylococcus aureus ATCC 25923 | - |
ATCC25923 | - |
| 1.1.1.100 | Streptococcus pneumoniae | - |
- |
- |
| 1.1.1.100 | Streptococcus sp. | - |
alpha-haemolytic strain 32213-7 and beta-haemolytic strain 32210-20 | - |
| 1.1.1.100 | synthetic construct | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.1.100 | by nickel chelation affinity chromatography | synthetic construct |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.1.1.100 | -80°C, 50% glycerol | synthetic construct |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.100 | ethyl acetoacetate + NADPH | - |
synthetic construct | ethyl 3-hydroxybutyrate + NADP+ | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.1.1.100 | beta-ketoacyl-ACP reductase | - |
Staphylococcus aureus |
| 1.1.1.100 | beta-ketoacyl-ACP reductase | - |
Pseudomonas aeruginosa |
| 1.1.1.100 | beta-ketoacyl-ACP reductase | - |
Streptococcus pneumoniae |
| 1.1.1.100 | beta-ketoacyl-ACP reductase | - |
Streptococcus sp. |
| 1.1.1.100 | beta-ketoacyl-ACP reductase | - |
synthetic construct |
| 1.1.1.100 | FabG | - |
Staphylococcus aureus |
| 1.1.1.100 | FabG | - |
Pseudomonas aeruginosa |
| 1.1.1.100 | FabG | - |
Streptococcus pneumoniae |
| 1.1.1.100 | FabG | - |
Streptococcus sp. |
| 1.1.1.100 | FabG | - |
synthetic construct |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.100 | 7 | - |
assay conditions | synthetic construct |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.100 | NADPH | - |
synthetic construct | |
IC50 Value
| EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.100 | additional information | - |
IC(50) value of 4.47 microg/ml for extract of galangal, pH 7.0 | synthetic construct | additional information | |
| 1.1.1.100 | 0.0212 | - |
pH 7.0 | synthetic construct | kaempferol | |
| 1.1.1.100 | 0.3048 | - |
pH 7.0 | synthetic construct | quercetin | |
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Release 2023.1 (January 2023)
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