Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Guterl, J.K.
    Andexer, J.N.; Sehl, T.; von Langermann, J.; Frindi-Wosch, I.; Rosenkranz, T.; Fitter, J.; Gruber, K.; Kragl, U.; Eggert, T.; Pohl, M.: Uneven twins: comparison of two enantiocomplementary hydroxynitrile lyases with alpha/beta-hydrolase fold (2009), J. Biotechnol., 141, 166-173.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.2.10 expression in Escherichia coli Arabidopsis thaliana
4.1.2.47 expressed in Escherichia coli BL21(DE3) cells Manihot esculenta
4.1.2.47 expression in Escherichia coli Manihot esculenta

Protein Variants

EC Number Protein Variants Comment Organism
4.1.2.47 C81A the mutant is also inhibited by acetate Manihot esculenta

General Stability

EC Number General Stability Organism
4.1.2.10 the enzyme is stabilized by addition of sorbitol and saccharose Arabidopsis thaliana
4.1.2.47 HNL is stabilized by entrapment in aggregates of Escherichia coli cell proteins Manihot esculenta
4.1.2.47 the enzyme is stabilized in crude cell extract Manihot esculenta

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.2.10 additional information no inhibition by acetate Arabidopsis thaliana
4.1.2.47 acetate
-
 Hevea brasiliensis
4.1.2.47 acetate
-
 Manihot esculenta
4.1.2.47 additional information no inhibition by acetate Manihot esculenta

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.10 1.4
-
 (R)-4-hydroxymandelonitrile pH 5.0, 25°C Arabidopsis thaliana
4.1.2.10 6
-
 benzaldehyde pH 6.0 Arabidopsis thaliana
4.1.2.10 6
-
 4-hydroxybenzaldehyde pH 5.0, 25°C Arabidopsis thaliana
4.1.2.11 4.1
-
 (S)-4-hydroxymandelonitrile pH 5.0, 25°C Manihot esculenta
4.1.2.11 5.9
-
 4-hydroxybenzaldehyde pH 5.0, 25°C Manihot esculenta
4.1.2.11 179
-
 cyanide pH 5.0, 25°C Manihot esculenta
4.1.2.47 4.1
-
 (S)-mandelonitrile pH 7.5 Manihot esculenta
4.1.2.47 4.1
-
 (S)-mandelonitrile citrate buffer (50 mM, pH 4.0), at 25°C Manihot esculenta
4.1.2.47 5.9
-
 benzaldehyde pH 7.5 Manihot esculenta
4.1.2.47 5.9
-
 benzaldehyde citrate buffer (50 mM, pH 4.0), at 25°C Manihot esculenta
4.1.2.47 179
-
 cyanide pH 7.5 Manihot esculenta
4.1.2.47 179
-
 cyanide citrate buffer (50 mM, pH 4.0), at 25°C Manihot esculenta

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.2.47 29200
-
 4 * 29200, in solution, calculated from amino acid sequence Hevea brasiliensis
4.1.2.47 29200
-
 4 * 29200, in solution, calculated from amino acid sequence Manihot esculenta
4.1.2.47 124000
-
 gel filtration Manihot esculenta

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.2.47 Acetone cyanhydrin Manihot esculenta
-
 Cyanide + acetone
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.10 Arabidopsis thaliana
-
-
-
4.1.2.11 Manihot esculenta
-
-
-
4.1.2.47 Hevea brasiliensis
-
-
-
4.1.2.47 Manihot esculenta
-
-
-
4.1.2.47 Manihot esculenta P52705
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.10 recombinant enzyme Arabidopsis thaliana
4.1.2.47 Q-Sepharose column chromatography Manihot esculenta
4.1.2.47 recombinant enzyme Manihot esculenta

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.10 (R)-4-hydroxymandelonitrile
-
 Arabidopsis thaliana cyanide + 4-hydroxybenzaldehyde
-
 r
4.1.2.10 (R)-mandelonitrile
-
 Arabidopsis thaliana cyanide + benzaldehyde
-
 r
4.1.2.10 cyanide + 4-hydroxybenzaldehyde
-
 Arabidopsis thaliana (R)-4-hydroxymandelonitrile
-
 r
4.1.2.10 additional information low activity towards acetone cyanohydrin Arabidopsis thaliana ?
-
 ?
4.1.2.11 (S)-4-Hydroxymandelonitrile
-
 Manihot esculenta Cyanide + 4-hydroxybenzaldehyde
-
 r
4.1.2.11 cyanide + 4-hydroxybenzaldehyde
-
 Manihot esculenta (S)-4-hydroxymandelonitrile
-
 r
4.1.2.47 (S)-mandelonitrile
-
 Hevea brasiliensis cyanide + benzaldehyde
-
 r
4.1.2.47 (S)-mandelonitrile
-
 Manihot esculenta cyanide + benzaldehyde
-
 r
4.1.2.47 Acetone cyanhydrin
-
 Manihot esculenta Cyanide + acetone
-
 ?
4.1.2.47 cyanide + benzaldehyde
-
 Manihot esculenta (S)-mandelonitrile
-
 r
4.1.2.47 cyclohexanone cyanohydrin
-
 Manihot esculenta ?
-
 ?
4.1.2.47 lactonitrile poor substrate Manihot esculenta ?
-
 ?
4.1.2.47 m-phenoxybenzaldehyde cyanohydrin 2-phenoxybenzaldehyde cyanohydrin is converted with lower activity than (S)-mandelonitrile and cyclohexanone cyanohydrile Manihot esculenta m-phenoxybenzaldehyde + cyanide
-
 ?
4.1.2.47 p-hydroxymandelonitrile phydroxymandelonitrile is converted with lower activity than (S)-mandelonitrile and cyclohexanone cyanohydrile Manihot esculenta ?
-
 ?
4.1.2.47 propionaldehyde cyanohydrin poor substrate Manihot esculenta propionaldehyde + cyanide
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.2.10 dimer the enzyme forms dimers in solution Arabidopsis thaliana
4.1.2.47 homotetramer 4 * 29200, in solution, calculated from amino acid sequence Hevea brasiliensis
4.1.2.47 homotetramer 4 * 29200, in solution, calculated from amino acid sequence Manihot esculenta
4.1.2.47 tetramer the enzyme forms dimers in solution Manihot esculenta

Synonyms

EC Number Synonyms Comment Organism
4.1.2.10 AtHNL
-
 Arabidopsis thaliana
4.1.2.10 R-hydroxynitrile lyase
-
 Arabidopsis thaliana
4.1.2.47 MeHNL
-
 Manihot esculenta
4.1.2.47 S-HnL
-
 Hevea brasiliensis
4.1.2.47 S-HnL
-
 Manihot esculenta
4.1.2.47 S-hydroxynitrile lyase
-
 Manihot esculenta
4.1.2.47 S-selective HnL
-
 Hevea brasiliensis
4.1.2.47 S-selective HnL
-
 Manihot esculenta

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.2.10 35
-
-
 Arabidopsis thaliana
4.1.2.11 60
-
-
 Manihot esculenta
4.1.2.47 60
-
-
 Manihot esculenta

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.2.10
-
 20 stable Arabidopsis thaliana
4.1.2.10 10
-
 half-life time: more than 96 h Arabidopsis thaliana
4.1.2.10 20
-
 half-life time: 80 h Arabidopsis thaliana
4.1.2.10 30
-
 half-life time: 33 h Arabidopsis thaliana
4.1.2.10 37
-
 half-life time: 6.6 h Arabidopsis thaliana
4.1.2.10 37
-
 half-life 6.6 h Arabidopsis thaliana
4.1.2.10 50
-
 half-life time: 0.3 h Arabidopsis thaliana
4.1.2.10 50
-
 half-life 0.3 h Arabidopsis thaliana
4.1.2.11
-
 20 stable Manihot esculenta
4.1.2.11 37
-
 half-life 64 h Manihot esculenta
4.1.2.11 50
-
 half-life 2.7 h Manihot esculenta
4.1.2.11 60
-
 half-life 0.5 h Manihot esculenta
4.1.2.47
-
 60 the enzyme is stable and exhibits more than 96 h half-life time between 0 and 20°C, the enzyme shows more than 48 h half-life time at 30°C, 64 h half-life time at 37°C, 2.7 h half-life time at 50°C, and 0.5 h half-life time at 60°C Manihot esculenta
4.1.2.47 20
-
 half-life time: more than 96 h Manihot esculenta
4.1.2.47 37
-
 half-life time: 64 h Manihot esculenta
4.1.2.47 40 50 the enzyme shows a half-life time of 4–11 h at 40°C. At 50°C a rapid inactivation is observed Hevea brasiliensis
4.1.2.47 50
-
 half-life time: 2.7 h Manihot esculenta
4.1.2.47 60
-
 half-life time: 30 min Manihot esculenta

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.2.10 5.7 6.5
-
 Arabidopsis thaliana
4.1.2.10 5.8 6.5
-
 Arabidopsis thaliana
4.1.2.11 5.7
-
-
 Manihot esculenta
4.1.2.47 5.8
-
-
 Hevea brasiliensis
4.1.2.47 5.8
-
-
 Manihot esculenta

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.2.10 5
-
 almost inactive below Arabidopsis thaliana
4.1.2.11 4
-
 40% of maxinmum activity Manihot esculenta
4.1.2.47 4 5.8 pH 4: about 40% of maximal activity, pH 5.8: optimum Manihot esculenta

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.1.2.10 5.4
-
 half-life time: 2 h, the deactivation of the enzyme at slightly acidic pH is a result of pronounced structural unfolding Arabidopsis thaliana
4.1.2.47 4
-
 half-life time: 2 h, the deactivation of the enzyme at slightly acidic pH is a result of pronounced structural unfolding Manihot esculenta
4.1.2.47 4
-
 the enzyme shows a half-life time of ca. 4 h at pH 5.0 Hevea brasiliensis
4.1.2.47 4
-
 the enzyme shows still 40% of its maximal activity at pH 4.0, while its activity is drastically reduced below pH 4.0 Manihot esculenta

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.1.2.10 Arabidopsis thaliana isoelectric focusing
-
 5.3
4.1.2.47 Hevea brasiliensis isoelectric focusing
-
 5.3
4.1.2.47 Manihot esculenta isoelectric focusing
-
 5.3