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Literature summary extracted from

  • Jerga, A.; Miller, D.J.; White, S.W.; Rock, C.O.
    Molecular determinants for interfacial binding and conformational change in a soluble diacylglycerol kinase (2009), J. Biol. Chem., 284, 7246-7254.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.107 dioleoyl-phosphatidylglycerol
-
 Staphylococcus aureus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.107 expressed in Escherichia coli BL21(DE3) cells Staphylococcus aureus

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.107 D124A mutant shows strongly reduced activity Staphylococcus aureus
2.7.1.107 D216A mutant shows strongly reduced activity Staphylococcus aureus
2.7.1.107 D271A mutant shows almost no activity Staphylococcus aureus
2.7.1.107 D68A mutant shows almost no activity Staphylococcus aureus
2.7.1.107 D97A mutant shows strongly reduced activity Staphylococcus aureus
2.7.1.107 E273A mutant shows almost no activity Staphylococcus aureus
2.7.1.107 K15A mutant shows reduced activity Staphylococcus aureus
2.7.1.107 K15A/K165A mutant shows strongly reduced activity Staphylococcus aureus
2.7.1.107 K165A mutant shows reduced activity Staphylococcus aureus
2.7.1.107 N96A mutant shows strongly reduced activity Staphylococcus aureus
2.7.1.107 R100A mutant shows reduced activity Staphylococcus aureus
2.7.1.107 R20A mutant shows wild type activity Staphylococcus aureus
2.7.1.107 T94A mutant shows strongly reduced activity Staphylococcus aureus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.107 soluble
-
 Staphylococcus aureus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.107 Mg2+ required for activity, interfacial binding of DgkB requires a Mg2+-dependent conformational change Staphylococcus aureus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.107 Staphylococcus aureus Q6GFF9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.107 Ni-NTA column chromatography Staphylococcus aureus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.107 0.0004
-
 mutant enzyme D271A, specific activity is less than 0.0004 micromol/min/mg, in 50 mM HEPES, pH 7.4, 150 mM LiCl, 10 mM MgCl2, 5 mM ATP, and lipid vesicles Staphylococcus aureus
2.7.1.107 0.0004
-
 mutant enzyme D68A, specific activity is less than 0.0004 micromol/min/mg, in 50 mM HEPES, pH 7.4, 150 mM LiCl, 10 mM MgCl2, 5 mM ATP, and lipid vesicles Staphylococcus aureus
2.7.1.107 0.0004
-
 mutant enzyme E273A, specific activity is less than 0.0004 micromol/min/mg, in 50 mM HEPES, pH 7.4, 150 mM LiCl, 10 mM MgCl2, 5 mM ATP, and lipid vesicles Staphylococcus aureus
2.7.1.107 0.0023
-
 mutant enzyme N96A, in 50 mM HEPES, pH 7.4, 150 mM LiCl, 10 mM MgCl2, 5 mM ATP, and lipid vesicles Staphylococcus aureus
2.7.1.107 0.0024
-
 mutant enzyme D124A, in 50 mM HEPES, pH 7.4, 150 mM LiCl, 10 mM MgCl2, 5 mM ATP, and lipid vesicles Staphylococcus aureus
2.7.1.107 0.0028
-
 mutant enzyme K15A/K165A, in 50 mM HEPES, pH 7.4, 150 mM LiCl, 10 mM MgCl2, 5 mM ATP, and lipid vesicles Staphylococcus aureus
2.7.1.107 0.0031
-
 mutant enzyme T94A, in 50 mM HEPES, pH 7.4, 150 mM LiCl, 10 mM MgCl2, 5 mM ATP, and lipid vesicles Staphylococcus aureus
2.7.1.107 0.0036
-
 mutant enzyme D97A, in 50 mM HEPES, pH 7.4, 150 mM LiCl, 10 mM MgCl2, 5 mM ATP, and lipid vesicles Staphylococcus aureus
2.7.1.107 0.0062
-
 mutant enzyme D216A, in 50 mM HEPES, pH 7.4, 150 mM LiCl, 10 mM MgCl2, 5 mM ATP, and lipid vesicles Staphylococcus aureus
2.7.1.107 0.0122
-
 mutant enzyme K15A, in 50 mM HEPES, pH 7.4, 150 mM LiCl, 10 mM MgCl2, 5 mM ATP, and lipid vesicles Staphylococcus aureus
2.7.1.107 0.0139
-
 mutant enzyme K165A, in 50 mM HEPES, pH 7.4, 150 mM LiCl, 10 mM MgCl2, 5 mM ATP, and lipid vesicles Staphylococcus aureus
2.7.1.107 0.0168
-
 mutant enzyme R100A, in 50 mM HEPES, pH 7.4, 150 mM LiCl, 10 mM MgCl2, 5 mM ATP, and lipid vesicles Staphylococcus aureus
2.7.1.107 0.0249
-
 wild type enzyme, in 50 mM HEPES, pH 7.4, 150 mM LiCl, 10 mM MgCl2, 5 mM ATP, and lipid vesicles Staphylococcus aureus
2.7.1.107 0.0256
-
 mutant enzyme R20A, in 50 mM HEPES, pH 7.4, 150 mM LiCl, 10 mM MgCl2, 5 mM ATP, and lipid vesicles Staphylococcus aureus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.107 ATP + 1,2-diacylglycerol
-
 Staphylococcus aureus ADP + 1,2-diacyl-sn-glycerol 3-phosphate
-
 ?
2.7.1.107 additional information 1,2-diacylglycerol embedded in unilamellar dioleoyl-phosphatidylcholine vesicles is not a substrate for DgkB Staphylococcus aureus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.107 DAG kinase
-
 Staphylococcus aureus
2.7.1.107 DgkB
-
 Staphylococcus aureus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.107 ATP
-
 Staphylococcus aureus