EC Number | Cloned (Comment) | Organism |
---|---|---|
5.5.1.16 | expressed in Escherichia coli strain C41 | Mycobacterium tuberculosis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
5.5.1.16 | (E,E,E)-geranylgeranyl diphosphate | substrate inhibition | Mycobacterium tuberculosis | |
5.5.1.16 | 15-aza-14,15-dihydrogeranylgeranyl diphosphate | - |
Mycobacterium tuberculosis | |
5.5.1.16 | 15-aza-14,15-dihydrogeranylgeranyl thiolodiphosphate | - |
Mycobacterium tuberculosis | |
5.5.1.16 | Mg2+ | the enzyme displays a rapid loss of activity as the Mg2+ concentration is raised above 0.1 mM | Mycobacterium tuberculosis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.5.1.16 | 0.0016 | - |
(E,E,E)-geranylgeranyl diphosphate | - |
Mycobacterium tuberculosis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
5.5.1.16 | Mg2+ | the enzyme requires Mg2+ and reacts most efficiently in the presence of low levels (0.1 mM) of Mg2+ | Mycobacterium tuberculosis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.5.1.16 | Mycobacterium tuberculosis | - |
- |
- |
5.5.1.16 | Mycobacterium tuberculosis H37Rv | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
5.5.1.16 | amylose resin column chromatography | Mycobacterium tuberculosis |
EC Number | Storage Stability | Organism |
---|---|---|
5.5.1.16 | 4°C, less than 24 h, the enzyme retains essentially full activity | Mycobacterium tuberculosis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.5.1.16 | (E,E,E)-geranylgeranyl diphosphate | - |
Mycobacterium tuberculosis | halimadienyl diphosphate | - |
? | |
5.5.1.16 | (E,E,E)-geranylgeranyl diphosphate | - |
Mycobacterium tuberculosis H37Rv | halimadienyl diphosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
5.5.1.16 | HPS | - |
Mycobacterium tuberculosis |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
5.5.1.16 | -4 | - |
freezing leads to loss of about 10% activity over the course of 1 week | Mycobacterium tuberculosis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.5.1.16 | 0.12 | - |
(E,E,E)-geranylgeranyl diphosphate | - |
Mycobacterium tuberculosis |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.5.1.16 | 0.018 | - |
(E,E,E)-geranylgeranyl diphosphate | - |
Mycobacterium tuberculosis |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
5.5.1.16 | 0.0000001 | - |
- |
Mycobacterium tuberculosis | 15-aza-14,15-dihydrogeranylgeranyl thiolodiphosphate | |
5.5.1.16 | 0.000002 | - |
- |
Mycobacterium tuberculosis | 15-aza-14,15-dihydrogeranylgeranyl diphosphate |
EC Number | General Information | Comment | Organism |
---|---|---|---|
5.5.1.16 | metabolism | class II diterpene cyclase that catalyzes the committed step in edaxadiene biosynthesis | Mycobacterium tuberculosis |