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Literature summary extracted from

  • Guss, A.M.; Kulkarni, G.; Metcalf, W.W.
    Differences in hydrogenase gene expression between Methanosarcina acetivorans and Methanosarcina barkeri (2009), J. Bacteriol., 191, 2826-2833.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.12.98.3 plasmid manipulation and isolation in Escherichia coli, promoter fusion plasmids are transformed into Methanosarcina acetivorans WWM82 via liposome-mediated transformation Methanosarcina acetivorans
1.12.98.3 plasmid manipulation and isolation in Escherichia coli, promoter fusion plasmids are transformed into Methanosarcina barkeri WWM85 via liposome-mediated transformation Methanosarcina barkeri

Organism

EC Number Organism UniProt Comment Textmining
1.12.98.3 Methanosarcina acetivorans
-
-
-
1.12.98.3 Methanosarcina barkeri
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.98.3 additional information growth on H2-CO2 Methanosarcina barkeri ?
-
?
1.12.98.3 additional information no growth on H2-CO2, inability to metabolize hydrogen, the lack of hydrogenase activity in Methanosarcina acetivorans extracts is not due to obvious mutations in either the coding regions or the promoters, hydrogenases are inactivated at the level of transcription and/or translation, Methanosarcina acetivorans possesses the machinery to express hydrogenases, although it does not express its own hydrogenases. This is consistent with specific inactivation of the Methanosarcina acetivorans hydrogenase promoters and highlight the importance of testing hypotheses generated by using genomic data Methanosarcina acetivorans ?
-
?

Synonyms

EC Number Synonyms Comment Organism
1.12.98.3 F420 non-reducing hydrogenase
-
Methanosarcina barkeri
1.12.98.3 methanophenazine-reducing hydrogenase
-
Methanosarcina barkeri
1.12.98.3 methanophenazine-reducing hydrogenase
-
Methanosarcina acetivorans
1.12.98.3 viologen-reducing hydrogenase
-
Methanosarcina barkeri