EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.100 | wild-type and mutants cloned into the pET-28a(+) vector and expressed in Escherichia coli BL21 (DE3) codon plus | Plasmodium falciparum |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.1.1.100 | R187A | kinetic constants (Km and acyl-carrier protein-independent specific activities) remain largely unchanged with respect to wild-type. Exhibits very poor activity in the acyl-carrier protein-dependent spectroscopic assay. No inhibition by increasing concentrations of acyl-carrier protein. 3fold decreased affinity binding to acyl-carrier protein with respect to wild-type | Plasmodium falciparum |
1.1.1.100 | R187A/R230A | kinetic constants (Km and acyl-carrier protein-independent specific activities) remain largely unchanged with respect to wild-type. Exhibits very poor activity in the acyl-carrier protein-dependent spectroscopic assay. 5fold decreased affinity binding to acyl-carrier protein with respect to wild-type | Plasmodium falciparum |
1.1.1.100 | R187E | kinetic constants (Km and acyl-carrier protein-independent specific activities) remain largely unchanged with respect to wild-type. Exhibits very poor activity in the acyl-carrier protein-dependent spectroscopic assay. No inhibition by increasing concentrations of acyl-carrier protein. 4fold decreased affinity binding to acyl-carrier protein with respect to wild-type | Plasmodium falciparum |
1.1.1.100 | R187E/R230E | kinetic constants (Km and acyl-carrier protein-independent specific activities) remain largely unchanged with respect to wild-type. Exhibits very poor activity in the acyl-carrier protein-dependent spectroscopic assay. 80fold decreased affinity binding to acyl-carrier protein with respect to wild-type | Plasmodium falciparum |
1.1.1.100 | R187K | no decreased affinity binding to acyl-carrier protein with respect to wild-type | Plasmodium falciparum |
1.1.1.100 | R230A | kinetic constants (Km and acyl-carrier protein-independent specific activities) remain largely unchanged with respect to wild-type. Exhibits very poor activity in the acyl-carrier protein-dependent spectroscopic assay. No inhibition by increasing concentrations of acyl-carrier protein. 5fold decreased affinity binding to acyl-carrier protein with respect to wild-type | Plasmodium falciparum |
1.1.1.100 | R230E | kinetic constants (Km and acyl-carrier protein-independent specific activities) remain largely unchanged with respect to wild-type. Exhibits very poor activity in the acyl-carrier protein-dependent spectroscopic assay. No inhibition by increasing concentrations of acyl-carrier protein. 41fold decreased affinity binding to acyl-carrier protein with respect to wild-type | Plasmodium falciparum |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.100 | acyl-carrier protein | inhibition of wild-type with increasing concentrations | Plasmodium falciparum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.100 | 0.43 | - |
acetoacetyl-CoA | wild-type | Plasmodium falciparum | |
1.1.1.100 | 0.44 | - |
acetoacetyl-CoA | mutant R230A | Plasmodium falciparum | |
1.1.1.100 | 0.45 | - |
acetoacetyl-CoA | mutant R187A | Plasmodium falciparum | |
1.1.1.100 | 0.47 | - |
acetoacetyl-CoA | mutant R187A/R230A | Plasmodium falciparum | |
1.1.1.100 | 0.47 | - |
acetoacetyl-CoA | mutant R187E | Plasmodium falciparum | |
1.1.1.100 | 0.49 | - |
acetoacetyl-CoA | mutant R230E | Plasmodium falciparum | |
1.1.1.100 | 0.58 | - |
acetoacetyl-CoA | mutant R187E/R230E | Plasmodium falciparum |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.100 | 31000 | - |
4 * 31000, SDS-PAGE, wild-type and mutants | Plasmodium falciparum |
1.1.1.100 | 110000 | - |
gel filtration, wild-type and mutants | Plasmodium falciparum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.100 | Plasmodium falciparum | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.100 | wild-type and mutants purified to homogeneity using an Ni2+-nitrilotriacetic acid affinity column | Plasmodium falciparum |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.100 | 2 | - |
mutant R187E/R230E, acyl-carrier protein-dependent spectroscopic assay | Plasmodium falciparum |
1.1.1.100 | 3 | - |
mutant R187A/R230A, acyl-carrier protein-dependent spectroscopic assay | Plasmodium falciparum |
1.1.1.100 | 4 | - |
mutant R230E, acyl-carrier protein-dependent spectroscopic assay | Plasmodium falciparum |
1.1.1.100 | 6.1 | - |
mutant R230A, acyl-carrier protein-dependent spectroscopic assay | Plasmodium falciparum |
1.1.1.100 | 6.3 | - |
mutant R187E, acyl-carrier protein-dependent spectroscopic assay | Plasmodium falciparum |
1.1.1.100 | 6.9 | - |
mutant R187A, acyl-carrier protein-dependent spectroscopic assay | Plasmodium falciparum |
1.1.1.100 | 42.6 | - |
mutant R187E/R230E, acyl-carrier protein-independent spectroscopic assay | Plasmodium falciparum |
1.1.1.100 | 45.6 | - |
mutant R187A/R230A, acyl-carrier protein-independent spectroscopic assay | Plasmodium falciparum |
1.1.1.100 | 54.2 | - |
mutant R230E, acyl-carrier protein-independent spectroscopic assay | Plasmodium falciparum |
1.1.1.100 | 54.5 | - |
mutant R187E, acyl-carrier protein-independent spectroscopic assay | Plasmodium falciparum |
1.1.1.100 | 55.3 | - |
mutant R230A, acyl-carrier protein-independent spectroscopic assay | Plasmodium falciparum |
1.1.1.100 | 57.9 | - |
mutant R187A, acyl-carrier protein-independent spectroscopic assay | Plasmodium falciparum |
1.1.1.100 | 59.8 | - |
wild-type, acyl-carrier protein-independent spectroscopic assay | Plasmodium falciparum |
1.1.1.100 | 70.6 | - |
wild-type, acyl-carrier protein-dependent spectroscopic assay | Plasmodium falciparum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.100 | acetoacetyl-CoA + NADPH | wild-type shows less activity with acetoacetyl-CoA than with acetoacetyl-[acyl-carrier protein] | Plasmodium falciparum | ? + NADP+ | - |
? | |
1.1.1.100 | acetoacetyl-[acyl-carrier protein] + NADPH | Arg187 and Arg230 are critical residues for the FabG-acyl-carrier protein interactions, significance of the positively charged/hydrophobic patch located adjacent to the active site cavities of FabG for interactions with acyl carrier protein | Plasmodium falciparum | D-beta-hydroxybutyryl-[acyl-carrier protein] + NADP+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.100 | homotetramer | 4 * 31000, SDS-PAGE, wild-type and mutants | Plasmodium falciparum |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.100 | beta-ketoacyl acyl carrier protein reductase | - |
Plasmodium falciparum |
1.1.1.100 | FabG | - |
Plasmodium falciparum |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.100 | NADPH | 3fold enhancement in acyl-carrier protein binding to wild-type in the presence of NADPH | Plasmodium falciparum |