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Literature summary extracted from

  • Arvizu-Flores, A.A.; Aispuro-Hernandez, E.; Garcia-Orozco, K.D.; Varela-Romero, A.; Valenzuela-Soto, E.; Velazquez-Contreras, E.F.; Rojo-Dominguez, A.; Yepiz-Plascencia, G.; Maley, F.; Sotelo-Mundo, R.R.
    Functional identity of the active sites of crustacean and viral thymidylate synthases (2009), Comp. Biochem. Physiol. C, 150, 406-413.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.45 expressed in Escherichia coli BL21(DE3) cells Shrimp white spot syndrome virus
2.1.1.45 expressed in Escherichia coli BL21(DE3) cells Penaeus vannamei

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.45 (S)-2-(5(((1,2-dihydro-3-methyl-1-oxobenzo[f]quinazolin-9 yl)methyl)amino)1-oxo-2-isoindolinyl)glutaric acid
-
 Penaeus vannamei
2.1.1.45 (S)-2-(5(((1,2-dihydro-3-methyl-1-oxobenzo[f]quinazolin-9-yl)methyl)amino)1-oxo-2-isoindolinyl)glutaric acid
-
 Shrimp white spot syndrome virus
2.1.1.45 10-propargyl-5,8-dideazafolate
-
 Penaeus vannamei
2.1.1.45 10-propargyl-5,8-dideazafolate
-
 Shrimp white spot syndrome virus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.45 0.0012
-
 dUMP at 30°C Shrimp white spot syndrome virus
2.1.1.45 0.0021
-
 dUMP at 30°C Penaeus vannamei
2.1.1.45 0.0134
-
 5,10-methylenetetrahydrofolate at 30°C Shrimp white spot syndrome virus
2.1.1.45 0.0135
-
 5,10-methylenetetrahydrofolate at 30°C Penaeus vannamei

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.45 Penaeus vannamei C6GJB8
-
-
2.1.1.45 Shrimp white spot syndrome virus Q8QTF3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.45 ammonium sulfate precipitation and Q-Sepharose column chromatography Shrimp white spot syndrome virus
2.1.1.45 ammonium sulfate precipitation and Q-Sepharose column chromatography Penaeus vannamei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Shrimp white spot syndrome virus dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Penaeus vannamei dihydrofolate + dTMP
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.45 ThyA
-
 Shrimp white spot syndrome virus
2.1.1.45 ThyA
-
 Penaeus vannamei

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.45 2.8
-
 5,10-methylenetetrahydrofolate at 30°C Shrimp white spot syndrome virus
2.1.1.45 4.1
-
 5,10-methylenetetrahydrofolate at 30°C Penaeus vannamei

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.45 N5,N10-methylenetetrahydrofolate
-
 Shrimp white spot syndrome virus
2.1.1.45 N5,N10-methylenetetrahydrofolate
-
 Penaeus vannamei

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.1.1.45 0.00015
-
 at 30°C Penaeus vannamei (S)-2-(5(((1,2-dihydro-3-methyl-1-oxobenzo[f]quinazolin-9 yl)methyl)amino)1-oxo-2-isoindolinyl)glutaric acid
2.1.1.45 0.00017
-
 at 30°C Penaeus vannamei 10-propargyl-5,8-dideazafolate
2.1.1.45 0.00019
-
 at 30°C Shrimp white spot syndrome virus (S)-2-(5(((1,2-dihydro-3-methyl-1-oxobenzo[f]quinazolin-9 yl)methyl)amino)1-oxo-2-isoindolinyl)glutaric acid
2.1.1.45 0.00023
-
 at 30°C Shrimp white spot syndrome virus 10-propargyl-5,8-dideazafolate

General Information

EC Number General Information Comment Organism
2.1.1.45 physiological function thymidylate synthase is the only de novo source of dTMP and is essential for DNA replication Shrimp white spot syndrome virus
2.1.1.45 physiological function thymidylate synthase is the only de novo source of dTMP and is essential for DNA replication Penaeus vannamei