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Literature summary extracted from

  • Ho, P.C.; Gupta, P.; Tsui, Y.C.; Ha, S.G.; Huq, M.; Wei, L.N.
    Modulation of lysine acetylation-stimulated repressive activity by Erk2-mediated phosphorylation of RIP140 in adipocyte differentiation (2008), Cell. Signal., 20, 1911-1919.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.32 additional information the phosphorylation of the receptor-interacting protein 140 (RIP140) by extracellular-signal-related kinase 2 (Erk2) stimulates p300 acetyltransferase to acetylate the RIP140's lysine 158 and lysine 287 residues Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.32 transfection of COS-1 cells (African green monkey cell line) with plasmids of wild-type enzyme, deficient enzyme or single site mutants fused to reporter genes Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.32 additional information Lysine 158 and 287 in the RIP140 (receptor-interacting protein 140) are targets for acetylation by p300 acetyltransferase as shown by site directed mutagenesis of these residues, lysine 158 and 287 replaced by glutamine (mimicking acetylation) turn the RIP140 repressive in the absence of p300, while the alanine replacements (preventing acetylation) prevents responsiveness to p300, the double mutants show stronger effects in the same directions, while lysine 111 and 311 seem not to be targets for the p300 acetyltransferase. Mitogen-activated protein kinase (MAPK) target mutants that mimic (T202E/T207E) or abolish (T202A/T207A) threonine phosphorylation of RIP140 enhance or reduce the stimulation of p300 acetyltransferase, respectively, extracellular-signal-related kinase 2 (Erk2) is identified as the responsible MAPK in this pathway Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.32 anacardic acid
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.32 acetyl-CoA + L-lysine Homo sapiens
-
 CoA + N6-acetyl-L-lysine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.32 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.32 cells are washed with PBS, harvested in Tris-HCl, pH 7.4, containing 0.5% deoxycholic acid, 150 mM NaCl, 0.1% SDS, 4 mM EDTA, and 1% NP-40, with a protease inhibitor cocktail, 1 mM PMSF, 1 mM sodium fluoride, and 1 mM sodium orthovanadate, centrifugation, supernatant subjected to SDS-PAGE, or for immunoprecipitation lysed cells are collected with 50 mM Tris-HCl, pH 8.0, with 10% glycerol, 100 mM NaCl, 1 mM EDTA, and 0.1% NP-40 with proteinase inhibitor cocktail, 1 mM PMSF, 1 mM sodium fluoride, and 1 mM sodium orthovanadate, incubation with antibodies and G beads, washing, elution of proteins by boiling in 2X Laemmli loading buffer Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.32 acetyl-CoA + L-lysine
-
 Homo sapiens CoA + N6-acetyl-L-lysine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.32 lysine acetyltransferase
-
 Homo sapiens
2.3.1.32 p300 acetyltransferase
-
 Homo sapiens

General Information

EC Number General Information Comment Organism
2.3.1.32 physiological function specific lysine 158 and lysine 287 acetylation of RIP140 (receptor-interacting protein 140) which is a co-regulator for many transcription factors, acetylation directly enhances the regulator's trans-repressive activity, role in fat accumulation Homo sapiens