EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.4.3.1 | additional information | in the sialyltransferase activity assay, FLAG-tagged ST6Gal1 has the highest expression level, which is 1.8- or 4.1fold higher than that of Strep- or His-tagged ST6Gal1, respectively | Rattus norvegicus |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.3.1 | ST6Gal1 cloned as N-terminal His-, Strep- and FLAG-tagged fusion proteins. FLAG-tagged recombinant ST6Gal1 expressed in fifth instar silkworm larval hemolymph using recombinant both cysteine protease- and chitinase-deficient Bombyx mori nucleopolyhedrovirus (BmNPV-CP--Chi-) bacmid | Rattus norvegicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.3.1 | 0.92 | - |
5-(5-dimethylaminonaphthalene-1-sulfonyl-2-(2-aminoethoxy))ethyl beta-N-acetyllactosamine | - |
Rattus norvegicus | |
2.4.3.1 | 2.8 | - |
5-(5-dimethylaminonaphthalene-1-sulfonyl-2-(2-aminoethoxy))ethyl beta-lactose | - |
Rattus norvegicus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.4.3.1 | MnCl2 | - |
Rattus norvegicus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.4.3.1 | 40000 | - |
1 * 40000, SDS-PAGE | Rattus norvegicus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.3.1 | Rattus norvegicus | P13721 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.3.1 | FLAG-tagged recombinant ST6Gal1 purified by precipitation with ammonium sulphate followed by affinity chromatography, with a recovery yield of 64%, 159fold | Rattus norvegicus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.4.3.1 | 0.016 | - |
crude enzyme | Rattus norvegicus |
2.4.3.1 | 2.6 | - |
159fold purified enzyme | Rattus norvegicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.3.1 | CMP-N-acetylneuraminate + 5-(5-dimethylaminonaphthalene-1-sulfonyl-2-(2-aminoethoxy))ethyl beta-lactose | - |
Rattus norvegicus | CMP + ? | - |
? | |
2.4.3.1 | CMP-N-acetylneuraminate + 5-(5-dimethylaminonaphthalene-1-sulfonyl-2-(2-aminoethoxy))ethyl beta-N-acetyllactosamine | - |
Rattus norvegicus | CMP + 5-(5-dimethylaminonaphthalene-1-sulfonyl-2-(2-aminoethoxy)) ethyl beta-N-acetylneuraminic acid alpha-2,6-N-acetyllactosamine | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.4.3.1 | monomer | 1 * 40000, SDS-PAGE | Rattus norvegicus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.3.1 | alpha2,6-sialyltransferase | - |
Rattus norvegicus |
2.4.3.1 | ST6Gal1 | - |
Rattus norvegicus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.3.1 | 37 | - |
purified enzyme | Rattus norvegicus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.3.1 | 20 | - |
purified enzyme is stable below 20°C but is rapidly inactivated at temperatures above 20°C | Rattus norvegicus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.3.1 | 0.087 | - |
5-(5-dimethylaminonaphthalene-1-sulfonyl-2-(2-aminoethoxy))ethyl beta-lactose | - |
Rattus norvegicus | |
2.4.3.1 | 1.13 | - |
5-(5-dimethylaminonaphthalene-1-sulfonyl-2-(2-aminoethoxy))ethyl beta-N-acetyllactosamine | - |
Rattus norvegicus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.4.3.1 | 6 | - |
purified enzyme | Rattus norvegicus |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
2.4.3.1 | 5 | 9 | purified enzyme is relatively stable over a wide range of pH | Rattus norvegicus |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
2.4.3.1 | Rattus norvegicus | isoelectric focusing | - |
6.3 |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.4.3.1 | physiological function | recombinant ST6Gal1 can be used as a key enzyme for the synthesis of an artificial sialoglycopolymer for potentially blocking influenza virus infection | Rattus norvegicus |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.3.1 | 0.031 | - |
5-(5-dimethylaminonaphthalene-1-sulfonyl-2-(2-aminoethoxy))ethyl beta-lactose | - |
Rattus norvegicus | |
2.4.3.1 | 1.23 | - |
5-(5-dimethylaminonaphthalene-1-sulfonyl-2-(2-aminoethoxy))ethyl beta-N-acetyllactosamine | - |
Rattus norvegicus |