EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
4.1.2.38 | dimethyl sulfoxide | highest activity between 20% and 30% dimethyl sulfoxide (v/v) with a peak at 25%. Records of pH-activity profiles at different dimethyl sulfoxide concentrations and constant ionic strength (100 mM) reveals a strong influence of the cosolvent on the activity of BAL, and indicates a cross effect on the optimal pH with a shift of profiles to the alkaline milieu. Correlation between the dependency on pH and dimethyl sulfoxide concentration is due to interaction of dimethyl sulfoxide with the amino acid side chain Glu50 in the catalytic site of the biocatalyst | Pseudomonas fluorescens | |
4.1.2.38 | additional information | synthetic BAL activity in KH2PO4 buffer (pH 8.5, 30% v/v dimethyl sulfoxide) reveals that the ionic strength of the buffer system has a very strong reciprocal effect on the activity of BAL, i.e. in a range between 25 mM and 500 mM, highest initial rates are observed at lowest ionic strength, and activity decreases with increasing ionic strength | Pseudomonas fluorescens |
EC Number | Cloned (Comment) | Organism |
---|---|---|
4.1.2.38 | expressed in Escherichia coli from SG13009prep4 (pBAL-His6) | Pseudomonas fluorescens |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.1.2.38 | Mg2+ | - |
Pseudomonas fluorescens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.1.2.38 | Pseudomonas fluorescens | - |
biovar I | - |
EC Number | Storage Stability | Organism |
---|---|---|
4.1.2.38 | -20°C | Pseudomonas fluorescens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.2.38 | 3,5-dimethoxy-benzaldehyde + 3,5-dimethoxy-benzaldehyde | - |
Pseudomonas fluorescens | (R)-3,3',5,5'-tetramethoxy-benzoin | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.1.2.38 | BAL | - |
Pseudomonas fluorescens |
4.1.2.38 | benzaldehyde lyase | - |
Pseudomonas fluorescens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.1.2.38 | additional information | - |
increase of the optimal pH of BAL with increasing concentrations of dimethyl sulfoxide | Pseudomonas fluorescens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
4.1.2.38 | thiamine diphosphate | - |
Pseudomonas fluorescens |