EC Number | Cloned (Comment) | Organism |
---|---|---|
4.2.3.37 | His-tagged version expressed in Escherichia coli BL21(DE3) | Streptomyces coelicolor |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
4.2.3.37 | sitting drop vapor diffusion method | Streptomyces coelicolor |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.2.3.37 | D99N | coordinates to Mg2+ | Streptomyces coelicolor |
4.2.3.37 | F198A | major products: Z-gamma-bisabolene (24%), sesqusabine (20%) | Streptomyces coelicolor |
4.2.3.37 | F96A | major product: beta-farnesene (70%) | Streptomyces coelicolor |
4.2.3.37 | W203F | major product: Z-gamma-bisabolene (47%) | Streptomyces coelicolor |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.3.37 | 0.00071 | - |
(2E,6E)-farnesyl diphosphate | wild-type protein | Streptomyces coelicolor | |
4.2.3.37 | 0.00077 | - |
(2E,6E)-farnesyl diphosphate | F96A mutant protein | Streptomyces coelicolor | |
4.2.3.37 | 0.0012 | - |
(2E,6E)-farnesyl diphosphate | F198A mutant protein | Streptomyces coelicolor | |
4.2.3.37 | 0.00145 | - |
(2E,6E)-farnesyl diphosphate | F203F mutant protein | Streptomyces coelicolor |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.2.3.37 | Mg2+ | - |
Streptomyces coelicolor |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.3.37 | Streptomyces coelicolor | Q9K499 | - |
- |
4.2.3.37 | Streptomyces coelicolor A3(2) | Q9K499 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.2.3.37 | immobilized metal ion affinity chromatography (Co2+) | Streptomyces coelicolor |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.3.37 | (2E,6E)-farnesyl diphosphate | - |
Streptomyces coelicolor | (+)-epi-isozizaene + diphosphate | major product (79%) | ? | |
4.2.3.37 | (2E,6E)-farnesyl diphosphate | - |
Streptomyces coelicolor A3(2) | (+)-epi-isozizaene + diphosphate | major product (79%) | ? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.2.3.37 | epi-isozizaene synthase | - |
Streptomyces coelicolor |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.3.37 | 0.00024 | - |
(2E,6E)-farnesyl diphosphate | F96A mutant protein | Streptomyces coelicolor | |
4.2.3.37 | 0.0003 | - |
(2E,6E)-farnesyl diphosphate | F198A mutant protein | Streptomyces coelicolor | |
4.2.3.37 | 0.00034 | - |
(2E,6E)-farnesyl diphosphate | F203F mutant protein | Streptomyces coelicolor | |
4.2.3.37 | 0.045 | - |
(2E,6E)-farnesyl diphosphate | wild-type protein | Streptomyces coelicolor |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.3.37 | 0.23 | - |
(2E,6E)-farnesyl diphosphate | F203F mutant protein | Streptomyces coelicolor | |
4.2.3.37 | 0.25 | - |
(2E,6E)-farnesyl diphosphate | F198A mutant protein | Streptomyces coelicolor | |
4.2.3.37 | 0.31 | - |
(2E,6E)-farnesyl diphosphate | F96A mutant protein | Streptomyces coelicolor | |
4.2.3.37 | 63 | - |
(2E,6E)-farnesyl diphosphate | wild-type protein | Streptomyces coelicolor |