Literature summary extracted from
Mulder, D.W.; Ortillo, D.O.; Gardenghi, D.J.; Naumov, A.V.; Ruebush, S.S.; Szilagyi, R.K.; Huynh, B.; Broderick, J.B.; Peters, J.W.
Activation of HydA(DeltaEFG) requires a preformed [4Fe-4S] cluster (2009), Biochemistry, 48, 6240-6248.
Activating Compound
EC Number |
Activating Compound |
Comment |
Organism |
Structure |
---|
1.12.99.6 |
HydE |
from Clostridium acetobutylicum extracts, activation of HydADELTAEFG requires the presence of a preformed [4Fe-4S] cluster on HydA |
Chlamydomonas reinhardtii |
|
1.12.99.6 |
HydF |
from Clostridium acetobutylicum extracts, activation of HydADELTAEFG requires the presence of a preformed [4Fe-4S] cluster on HydA |
Chlamydomonas reinhardtii |
|
1.12.99.6 |
HydG |
from Clostridium acetobutylicum extracts, activation of HydADELTAEFG requires the presence of a preformed [4Fe-4S] cluster on HydA |
Chlamydomonas reinhardtii |
|
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
1.12.99.6 |
HydADELTAEFG cloned into a pET Duet vector and modified for the presence of an N-terminal six-histidine tag. Expressed in Escherichia coli BL21(DE3) cells |
Chlamydomonas reinhardtii |
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
1.12.99.6 |
Fe |
HydADELTAEFG binds a [4Fe-4S] cluster |
Chlamydomonas reinhardtii |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
1.12.99.6 |
49000 |
- |
x * 49000, SDS-PAGE, HydADELTAEFG |
Chlamydomonas reinhardtii |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.12.99.6 |
Chlamydomonas reinhardtii |
- |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
1.12.99.6 |
HydADELTAEFG purified by anion exchange chromatography |
Chlamydomonas reinhardtii |
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
1.12.99.6 |
? |
x * 49000, SDS-PAGE, HydADELTAEFG |
Chlamydomonas reinhardtii |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
1.12.99.6 |
hydA |
- |
Chlamydomonas reinhardtii |