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Literature summary extracted from

  • Bruning, M.; Berheide, M.; Meyer, D.; Golbik, R.; Bartunik, H.; Liese, A.; Tittmann, K.
    Structural and kinetic studies on native intermediates and an intermediate analogue in benzoylformate decarboxylase reveal a least motion mechanism with an unprecedented short-lived predecarboxylation intermediate (2009), Biochemistry, 48, 3258-3268.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.1.7 sitting drop vapor diffusion method, using 25% (w/v) polyethylene glycol 2000 and 0.2 M MgCl2, at pH 6. Pseudomonas putida

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.7 (R)-mandelate
-
 Pseudomonas putida
4.1.1.7 methyl benzoylphosphonate
-
 Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.7 0.15
-
 p-nitro-benzoylformate at 25°C Pseudomonas putida
4.1.1.7 0.23
-
 benzoylformate in 50 mM potassium phosphate buffer, pH 6.5, at 30°C Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.7 Pseudomonas putida P20906
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.7
-
 Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.7 benzoylformate
-
 Pseudomonas putida benzaldehyde + CO2
-
 ?
4.1.1.7 benzylformate
-
 Pseudomonas putida benzaldehyde + CO2
-
 ?
4.1.1.7 p-nitro-benzoylformate very poor substrate Pseudomonas putida ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.7 homotetramer x-ray crystallography Pseudomonas putida

Synonyms

EC Number Synonyms Comment Organism
4.1.1.7 BFD
-
 Pseudomonas putida

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.1.7 0.05
-
 p-nitro-benzoylformate at 25°C Pseudomonas putida
4.1.1.7 426
-
 benzoylformate at 30°C, in 50 mM potassium phosphate buffer, pH 6.5 Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.7 thiamine diphosphate
-
 Pseudomonas putida