EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.1.17 | overexpressed both as native and as fusion protein with a His6 tag at the C- or the N-terminus in human embryonic kidney-293 cells expressing the large T-antigen of simian virus 40 (HEK-293T cells) | Homo sapiens |
2.3.1.17 | overexpressed both as native and as fusion proteins with a His6 tag at the C- or the N-terminus in human embryonic kidney-293 cells expressing the large T-antigen of simian virus 40 (HEK-293T cells); furthermore expressed as fusion protein in rat embryonal cortical neurons and in Chinese-hamster ovary cells | Mus musculus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.3.1.17 | glutamate | Glutamate is a competitive inhibitor. No inhibition is observed with other amino acids, indicating that the enzyme is specific. | Mus musculus | |
2.3.1.17 | N-acetyl-L-aspartate | - |
Mus musculus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.17 | 0.009 | - |
acetyl-CoA | in presence of 0.5 mM CHAPS | Mus musculus | |
2.3.1.17 | 0.013 | - |
acetyl-CoA | in presence of 1 mM CHAPS | Mus musculus | |
2.3.1.17 | 0.09 | - |
L-aspartate | - |
Mus musculus | |
2.3.1.17 | 5 | - |
glutamate | - |
Mus musculus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.3.1.17 | endoplasmic reticulum | - |
Mus musculus | 5783 | - |
2.3.1.17 | additional information | not in mitochondria | Mus musculus | - |
- |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.17 | Danio rerio | - |
- |
- |
2.3.1.17 | Homo sapiens | Q8N9F0 | - |
- |
2.3.1.17 | Mus musculus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.1.17 | - |
Mus musculus |
2.3.1.17 | - |
Homo sapiens |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.3.1.17 | brain | - |
Mus musculus | - |
2.3.1.17 | brain | - |
Homo sapiens | - |
2.3.1.17 | additional information | In PCR, no specific amplification was observed with liver, kidney, lung, skeletal muscle, testis and heart cDNA of mice. | Mus musculus | - |
2.3.1.17 | spleen | - |
Mus musculus | - |
2.3.1.17 | thymus | - |
Mus musculus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.3.1.17 | 0.0015 | - |
activity of cloned mouse NAT8L fusion protein with N-terminal His6 tag | Mus musculus |
2.3.1.17 | 0.0018 | - |
activity of cloned mouse NAT8L fusion protein with C-terminal His6 tag | Mus musculus |
2.3.1.17 | 0.0025 | - |
activity of cloned wild type mouse NAT8L protein | Mus musculus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.17 | acetyl-CoA + glutamate | [14C]glutamate is acetylated with an approximately 50-fold lower affinity and a similar Vmax compared with aspartate | Mus musculus | CoA + N-acetylglutamate | - |
? | |
2.3.1.17 | acetyl-CoA + L-aspartate | - |
Mus musculus | CoA + N-acetyl-L-aspartic acid | - |
? | |
2.3.1.17 | acetyl-CoA + L-aspartate | - |
Homo sapiens | CoA + N-acetyl-L-aspartic acid | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.1.17 | aspartate N-acetyltransferase | - |
Mus musculus |
2.3.1.17 | aspartate N-acetyltransferase | - |
Danio rerio |
2.3.1.17 | aspartate N-acetyltransferase | - |
Homo sapiens |
2.3.1.17 | NAT | - |
Mus musculus |
2.3.1.17 | NAT | - |
Danio rerio |
2.3.1.17 | NAT | - |
Homo sapiens |
2.3.1.17 | NAT8L | - |
Mus musculus |
2.3.1.17 | NAT8L | - |
Homo sapiens |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.17 | 0.56 | - |
N-acetyl-L-aspartate | - |
Mus musculus | |
2.3.1.17 | 6.1 | - |
glutamate | - |
Mus musculus |