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Literature summary extracted from
- Expression of L-ornithine Ndelta-oxygenase (PvdA) in fluorescent Pseudomonas species: an immunochemical and in silico study (2004), Biochem. Biophys. Res. Commun., 313, 245-257.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.13.195 | PvdA, DNA and amino acid sequence determination and analysis, sequence comparisons | Pseudomonas fluorescens |
| 1.14.13.195 | PvdA, DNA and amino acid sequence determination and analysis, sequence comparisons | Pseudomonas putida |
| 1.14.13.195 | PvdA, DNA and amino acid sequence determination and analysis, sequence comparisons | Burkholderia cepacia |
| 1.14.13.195 | PvdA, DNA and amino acid sequence determination and analysis, sequence comparisons | Pseudomonas syringae |
| 1.14.13.195 | PvdA, DNA and amino acid sequence determination and analysis, sequence comparisons | Ralstonia solanacearum |
| 1.14.13.195 | PvdA, DNA and amino acid sequence determination and analysis, sequence comparisons, promoter activity analysis during cell growth. Recombinant expression of N-terminally His-tagged full-length and truncated wild-type and mutant enzymes in Escherichia coli strain M15 | Pseudomonas aeruginosa |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.14.13.195 | additional information | construction of diverse inactive truncation mutants, overview | Pseudomonas aeruginosa |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.13.195 | L-ornithine + NADPH + H+ + O2 | Pseudomonas fluorescens | first step in pyoverdine biosynthesis | N5-hydroxy-L-ornithine + NADP+ + H2O | - |
? |  |
| 1.14.13.195 | L-ornithine + NADPH + H+ + O2 | Pseudomonas putida | first step in pyoverdine biosynthesis | N5-hydroxy-L-ornithine + NADP+ + H2O | - |
? | |
| 1.14.13.195 | L-ornithine + NADPH + H+ + O2 | Burkholderia cepacia | first step in pyoverdine biosynthesis | N5-hydroxy-L-ornithine + NADP+ + H2O | - |
? | |
| 1.14.13.195 | L-ornithine + NADPH + H+ + O2 | Pseudomonas syringae | first step in pyoverdine biosynthesis | N5-hydroxy-L-ornithine + NADP+ + H2O | - |
? | |
| 1.14.13.195 | L-ornithine + NADPH + H+ + O2 | Ralstonia solanacearum | first step in pyoverdine biosynthesis | N5-hydroxy-L-ornithine + NADP+ + H2O | - |
? | |
| 1.14.13.195 | L-ornithine + NADPH + H+ + O2 | Pseudomonas aeruginosa | first step in pyoverdine biosynthesis | N5-hydroxy-L-ornithine + NADP+ + H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.13.195 | Burkholderia cepacia | - |
gene pvdA | - |
| 1.14.13.195 | Pseudomonas aeruginosa | Q51548 | several clinical isolates, gene pvdA | - |
| 1.14.13.195 | Pseudomonas fluorescens | - |
strains B10 and PF, gene pvdA | - |
| 1.14.13.195 | Pseudomonas putida | - |
strain KT2440, no activity in strain WCS358, gene pvdA | - |
| 1.14.13.195 | Pseudomonas syringae | - |
pv. syringae, strain B278a, gene pvdA | - |
| 1.14.13.195 | Ralstonia solanacearum | - |
gene pvdA | - |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 1.14.13.195 | flavoprotein | - |
Pseudomonas fluorescens |
| 1.14.13.195 | flavoprotein | - |
Pseudomonas putida |
| 1.14.13.195 | flavoprotein | - |
Burkholderia cepacia |
| 1.14.13.195 | flavoprotein | - |
Pseudomonas syringae |
| 1.14.13.195 | flavoprotein | - |
Ralstonia solanacearum |
| 1.14.13.195 | flavoprotein | - |
Pseudomonas aeruginosa |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.13.195 | recombinant N-terminally His-tagged full-length and truncated wild-type and mutant enzymes from Escherichia coli strain M15 by nickel affinity chromatography | Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.13.195 | L-ornithine + NADPH + H+ + O2 | - |
Pseudomonas fluorescens | N5-hydroxy-L-ornithine + NADP+ + H2O | - |
? | |
| 1.14.13.195 | L-ornithine + NADPH + H+ + O2 | - |
Pseudomonas putida | N5-hydroxy-L-ornithine + NADP+ + H2O | - |
? | |
| 1.14.13.195 | L-ornithine + NADPH + H+ + O2 | - |
Burkholderia cepacia | N5-hydroxy-L-ornithine + NADP+ + H2O | - |
? | |
| 1.14.13.195 | L-ornithine + NADPH + H+ + O2 | - |
Pseudomonas syringae | N5-hydroxy-L-ornithine + NADP+ + H2O | - |
? | |
| 1.14.13.195 | L-ornithine + NADPH + H+ + O2 | - |
Ralstonia solanacearum | N5-hydroxy-L-ornithine + NADP+ + H2O | - |
? | |
| 1.14.13.195 | L-ornithine + NADPH + H+ + O2 | - |
Pseudomonas aeruginosa | N5-hydroxy-L-ornithine + NADP+ + H2O | - |
? | |
| 1.14.13.195 | L-ornithine + NADPH + H+ + O2 | first step in pyoverdine biosynthesis | Pseudomonas fluorescens | N5-hydroxy-L-ornithine + NADP+ + H2O | - |
? | |
| 1.14.13.195 | L-ornithine + NADPH + H+ + O2 | first step in pyoverdine biosynthesis | Pseudomonas putida | N5-hydroxy-L-ornithine + NADP+ + H2O | - |
? | |
| 1.14.13.195 | L-ornithine + NADPH + H+ + O2 | first step in pyoverdine biosynthesis | Burkholderia cepacia | N5-hydroxy-L-ornithine + NADP+ + H2O | - |
? | |
| 1.14.13.195 | L-ornithine + NADPH + H+ + O2 | first step in pyoverdine biosynthesis | Pseudomonas syringae | N5-hydroxy-L-ornithine + NADP+ + H2O | - |
? | |
| 1.14.13.195 | L-ornithine + NADPH + H+ + O2 | first step in pyoverdine biosynthesis | Ralstonia solanacearum | N5-hydroxy-L-ornithine + NADP+ + H2O | - |
? | |
| 1.14.13.195 | L-ornithine + NADPH + H+ + O2 | first step in pyoverdine biosynthesis | Pseudomonas aeruginosa | N5-hydroxy-L-ornithine + NADP+ + H2O | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.13.195 | More | immunochemical peptide mapping, molecular weights calculated for the truncated enzyme fragments, overview | Pseudomonas aeruginosa |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.13.195 | L-ornithine-Ndelta-oxygenase | - |
Pseudomonas fluorescens |
| 1.14.13.195 | L-ornithine-Ndelta-oxygenase | - |
Pseudomonas putida |
| 1.14.13.195 | L-ornithine-Ndelta-oxygenase | - |
Burkholderia cepacia |
| 1.14.13.195 | L-ornithine-Ndelta-oxygenase | - |
Pseudomonas syringae |
| 1.14.13.195 | L-ornithine-Ndelta-oxygenase | - |
Ralstonia solanacearum |
| 1.14.13.195 | L-ornithine-Ndelta-oxygenase | - |
Pseudomonas aeruginosa |
| 1.14.13.195 | PvdA | - |
Pseudomonas fluorescens |
| 1.14.13.195 | PvdA | - |
Pseudomonas putida |
| 1.14.13.195 | PvdA | - |
Burkholderia cepacia |
| 1.14.13.195 | PvdA | - |
Pseudomonas syringae |
| 1.14.13.195 | PvdA | - |
Ralstonia solanacearum |
| 1.14.13.195 | PvdA | - |
Pseudomonas aeruginosa |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.13.195 | FAD | required, functions between NADPH and enzyme | Pseudomonas fluorescens | |
| 1.14.13.195 | FAD | required, functions between NADPH and enzyme | Pseudomonas putida | |
| 1.14.13.195 | FAD | required, functions between NADPH and enzyme | Burkholderia cepacia | |
| 1.14.13.195 | FAD | required, functions between NADPH and enzyme | Pseudomonas syringae | |
| 1.14.13.195 | FAD | required, functions between NADPH and enzyme | Ralstonia solanacearum | |
| 1.14.13.195 | FAD | required, functions between NADPH and enzyme | Pseudomonas aeruginosa | |
| 1.14.13.195 | NADPH | - |
Pseudomonas fluorescens | |
| 1.14.13.195 | NADPH | - |
Pseudomonas putida | |
| 1.14.13.195 | NADPH | - |
Burkholderia cepacia | |
| 1.14.13.195 | NADPH | - |
Pseudomonas syringae | |
| 1.14.13.195 | NADPH | - |
Ralstonia solanacearum | |
| 1.14.13.195 | NADPH | - |
Pseudomonas aeruginosa | |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 1.14.13.195 | Pseudomonas aeruginosa | pI values, calculated for the truncated enzyme fragments, overview | - |
additional information |
Expression
| EC Number | Organism | Comment | Expression |
|---|---|---|---|
| 1.14.13.195 | Pseudomonas fluorescens | the enzyme expression is induced by iron | up |
| 1.14.13.195 | Pseudomonas putida | the enzyme expression is induced by iron | up |
| 1.14.13.195 | Burkholderia cepacia | the enzyme expression is induced by iron | up |
| 1.14.13.195 | Pseudomonas syringae | the enzyme expression is induced by iron | up |
| 1.14.13.195 | Ralstonia solanacearum | the enzyme expression is induced by iron | up |
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