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Literature summary extracted from

  • Pedrolli, D.; Carmona, E.
    Pectin lyase from Aspergillus giganteus: Comparative study of productivity of submerged fermentation on citrus pectin and orange waste (2009), Appl. Biochem. Microbiol., 45, 610-616.
No PubMed abstract available

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.10 Aspergillus giganteus
-
-
-
4.2.2.10 Aspergillus giganteus CCT 3232
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.2.10 additional information
-
 the specific activity of pectin lyase using citrus pectin as substrate is 60.891 units/mg, the specific activity of pectin lyase using orange waste as substrate is 38.364 units/mg, the specific activity of pectin lyase using polygalacturonic acid as substrate is 35.86 units/mg, the specific activity of pectin lyase using galacturonic acid as substrate is 63.403 units/mg, one unit of activity is defined as the amount of enzyme causing a rise in 0.010 absorbance units per h Aspergillus giganteus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.10 citrus pectin
-
 Aspergillus giganteus ?
-
 ?
4.2.2.10 citrus pectin
-
 Aspergillus giganteus CCT 3232 ?
-
 ?
4.2.2.10 galacturonic acid
-
 Aspergillus giganteus ?
-
 ?
4.2.2.10 galacturonic acid
-
 Aspergillus giganteus CCT 3232 ?
-
 ?
4.2.2.10 orange waste under optimum conditions, the highest pectin lyase activity in the medium is with orange waste as carbon source Aspergillus giganteus ?
-
 ?
4.2.2.10 orange waste under optimum conditions, the highest pectin lyase activity in the medium is with orange waste as carbon source Aspergillus giganteus CCT 3232 ?
-
 ?
4.2.2.10 polygalacturonic acid
-
 Aspergillus giganteus ?
-
 ?
4.2.2.10 polygalacturonic acid
-
 Aspergillus giganteus CCT 3232 ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.10 50
-
-
 Aspergillus giganteus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.2.10 45 50 the pectin lyase activity shows thermal stability, with half-lives of 30 and 27 min when incubated at 45 and 50°C, respectively Aspergillus giganteus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.10 8.5 9
-
 Aspergillus giganteus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.2.2.10 6 10 high stability is observed at room temperature from pH 6.0 to 10.0 with more than 85% of enzyme activity preserved Aspergillus giganteus