Literature summary extracted from
Mavrodi, D.V.; Bleimling, N.; Thomashow, L.S.; Blankenfeldt, W.
The purification, crystallization and preliminary structural characterization of PhzF, a key enzyme in the phenazine-biosynthesis pathway from Pseudomonas fluorescens 2-79 (2004), Acta Crystallogr. Sect. D, 60, 184-186.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
5.3.3.17 |
large-scale expression in Escherichia coli |
Pseudomonas fluorescens |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
5.3.3.17 |
hanging-drop vapour-diffusion method. Crystallized from PEG 4000/ammonium sulfate/sodium citrate pH 5.6. The crystals belong to space group P3(1)21 or P3(2)21, with unit-cell parameters a = b = 56.3, c = 156.4 A. They contain one monomer in the asymmetric unit and diffract to better than 1.7 A on synchrotron beamlines. Crystals of seleno-L-methionine-labelled PhzF have been obtained |
Pseudomonas fluorescens |
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
5.3.3.17 |
32000 |
- |
two protein species with MW 32048 Da and 32224 Da, MALDI-TOF mass-spectrometry |
Pseudomonas fluorescens |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
5.3.3.17 |
Pseudomonas fluorescens |
Q51792 |
strain 2-79 |
- |
5.3.3.17 |
Pseudomonas fluorescens Feb 79 |
Q51792 |
strain 2-79 |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
5.3.3.17 |
- |
Pseudomonas fluorescens |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
5.3.3.17 |
phzF |
- |
Pseudomonas fluorescens |