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Literature summary extracted from

  • Nystedt, J.; Anderson, H.; Hirvonen, T.; Impola, U.; Jaatinen, T.; Heiskanen, A.; Blomquist, M.; Satomaa, T.; Natunen, J.; Saarinen, J.; Lehenkari, P.; Valmu, L.; Laine, J.
    Human CMP-N-acetylneuraminic acid hydroxylase (CMAH) is a novel stem cell marker linked to stem cell-specific mechanisms (2010), Stem Cells, 28, 258-267.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.14.18.2 medicine the results pinpoint the crucial need for totally xenofree culturing and expansion conditions for human therapeutic stem cells Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.18.2 full-length CMAH cDNA is cloned from umbilical cord blood mononuclear cells and HeLa cells into the pGEM-T easy vector for sequencing, GFP- or FLAG-tagged constructs are prepared using the vectors pFLAG-CMV-2 and pEGFP-C2/pEGFP-C1 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.18.2 cytoplasm
-
 Homo sapiens 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.18.2 75000
-
 determined by SDS-PAGE and Western Blot analysis Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.18.2 CMP-N-acetylneuraminate + ferrocytochrome b5 + O2 + H+ Homo sapiens
-
 CMP-N-glycoloylneuraminate + ferricytochrome b5 + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.18.2 Homo sapiens C1K3L2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.18.2 cell extracts are prepared Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.18.2 HEK-293 cell
-
 Homo sapiens
-
1.14.18.2 HeLa cell
-
 Homo sapiens
-
1.14.18.2 hematopoietic stem cell
-
 Homo sapiens
-
1.14.18.2 Huh-7 cell
-
 Homo sapiens
-
1.14.18.2 mesenchymal stem cell
-
 Homo sapiens
-
1.14.18.2 mononuclear cell
-
 Homo sapiens
-
1.14.18.2 umbilical cord blood
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.18.2 CMP-N-acetylneuraminate + ferrocytochrome b5 + O2 + H+
-
 Homo sapiens CMP-N-glycoloylneuraminate + ferricytochrome b5 + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.18.2 CMAH
-
 Homo sapiens
1.14.18.2 CMP-N-acetylneuraminic acid hydroxylase
-
 Homo sapiens