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Literature summary extracted from

  • Szarka, A.; Horemans, N.; Passarella, S.; Tarcsay, A.; Orsi, F.; Salgo, A.; Banhegyi, G.
    Demonstration of an intramitochondrial invertase activity and the corresponding sugar transporters of the inner mitochondrial membrane in Jerusalem artichoke (Helianthus tuberosus L.) tubers (2008), Planta, 228, 765-775.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.2.1.26 enzyme activity almost constant within investigated time range of 250 min at room temperature Helianthus tuberosus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.26 AgNO3 0.1 mM, 37% inhibition; 1 mM, 88% inhibition Helianthus tuberosus
3.2.1.26 CuSO4 0.1 mM, 46% inhibition; 1 mM, 100% inhibition Helianthus tuberosus
3.2.1.26 fructose 40 mM, 52% inhibition; 4 mM, 22% inhibition Helianthus tuberosus
3.2.1.26 glucose 40 mM, 100% inhibition Helianthus tuberosus
3.2.1.26 HgCl2 0.1 mM, 48% inhibition; 1 mM, 89% inhibition Helianthus tuberosus
3.2.1.26 additional information MgCl2 and CaCl2 (1 mM) are typical alkaline invertase inhibitors, they do not inhibit the neutral invertase Helianthus tuberosus
3.2.1.26 Tris 10 mM, 87% inhibition; 1 mM, 74% inhibition Helianthus tuberosus
3.2.1.26 ZnCl2 0.1 mM, 84% inhibition; 1 mM, 100% inhibition Helianthus tuberosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.26 21
-
 sucrose 37°C Helianthus tuberosus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.26 mitochondrial matrix highest invertase activities compared to inner or outer membrane, since transport capacity through matrix is limiting enzyme activity, enzyme must be located on inner matrix Helianthus tuberosus 5759
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.26 sucrose + H2O Helianthus tuberosus
-
 D-fructose + D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.26 Helianthus tuberosus
-
 Jerusalem artichoke
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.26 mitochondrial fraction isolated by several centrifugation steps from filtrate (cheesecloth) of ground and homogenized tubers, final centrifugation step with mannitol gradient (lower fraction excluding the bottom) followed by several washing steps of the pellet, resuspension in 10 mM morpholinepropanesulfonic acid + 70 mM mannitol buffer, pH 7.4, further centrifugation steps to separate mitoplast pellet from intermembrane space and outer membrane and separate the mitoplast pellet after repeated freeze-thawing rupture into matrix supernatant and inner membrane pellet, washed and resuspended in 10 mM morpholinepropanesulfonic acid buffer, pH 7.4 Helianthus tuberosus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.26 tuber
-
 Helianthus tuberosus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.26 0.0005
-
 inner membrane Helianthus tuberosus
3.2.1.26 0.0008
-
 outer membrane Helianthus tuberosus
3.2.1.26 0.0116
-
 matrix, 10 min, room temperature, control Helianthus tuberosus
3.2.1.26 0.014
-
 untreated mitochondria, 37°C Helianthus tuberosus
3.2.1.26 0.0196
-
 matrix Helianthus tuberosus
3.2.1.26 0.0231
-
 mitochondria, alamethicin treated to enable unrestricted transport, 37°C Helianthus tuberosus
3.2.1.26 0.0269
-
 matrix, 10 min, 37°C Helianthus tuberosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.26 sucrose + H2O
-
 Helianthus tuberosus D-fructose + D-glucose
-
 ?
3.2.1.26 sucrose + H2O 100 mM sucrose, 1 mg/ml mitochondria, room temperature (22-25°C), 300 mM mannitol + 10 mM morpholinepropanesulfonic acid buffer, pH 7.4, no enzyme activity with maltose or raffinose Helianthus tuberosus D-fructose + D-glucose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.26 neutral invertase
-
 Helianthus tuberosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.26 7.2
-
 pH adjusted with KOH or HCl Helianthus tuberosus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.26 5 8.5 less than 20% activity at pH 5, more than 80% activity between pH 6.2 and 7.7 with maximum at pH 7 and 7.2, 50-70% activity between pH 8 and 8.5 Helianthus tuberosus