EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.26 | Lin8 400 bp fragment inserted into plant transformation vector pK7GWIWG2(II), transformed into tomato plants via Agrobacterium tumefaciens-mediated gene transfer | Solanum lycopersicum |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.2.1.26 | cell wall | two isoforms: Lin6 and Lin8 | Solanum lycopersicum | 5618 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.26 | additional information | Solanum lycopersicum | function of cell wall-bound invertase activity during pathogen infection, the Lin8-mutant lines 33 and 57 do not increase activity in response to Xanthomonas campestris pv vesicatoria infection, line 50 only slightly, and all mutant lines show symptoms delayed, expression of senescent-associated genes is decreased, mutants have about 50% lower starch content due to 2-fold increased sucrose export, and show a tendency for increased fructose content | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.26 | Solanum lycopersicum | - |
var. Moneymaker wild-type and with silenced leaf isoforms of cw-Inv, interaction with Xanthomonas campestris pv vesicatoria infection | - |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.2.1.26 | leaf | - |
Solanum lycopersicum | - |
3.2.1.26 | additional information | tomato lines with strongly reduced source leaf cell wall-bound invertase activity by RNAi inhibition, 10-15% source leaf activity, while sink leaves and petioles still have 40-60% of wild-type activity, no alterations in other organs and tissue: Lin8-RNAi line 33, line 50, line 57, gene-silencing | Solanum lycopersicum | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.26 | additional information | - |
Lin8-RNAi line 33 activity: 3.4 micromol/squaremeter/min, vacuolar invertase: 3.6 micromol/squaremeter/min | Solanum lycopersicum |
3.2.1.26 | additional information | - |
Lin8-RNAi line 50 activity: 3.6 micromol/squaremeter/min, vacuolar invertase: 5.6 micromol/squaremeter/min | Solanum lycopersicum |
3.2.1.26 | additional information | - |
Lin8-RNAi line 57 activity: 1.5 micromol/squaremeter/min, vacuolar invertase: 4.0 micromol/squaremeter/min | Solanum lycopersicum |
3.2.1.26 | additional information | - |
wild-type activity: 40.5 micromol/squaremeter/min, vacuolar invertase: 4.2 micromol/squaremeter/min | Solanum lycopersicum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.26 | additional information | function of cell wall-bound invertase activity during pathogen infection, the Lin8-mutant lines 33 and 57 do not increase activity in response to Xanthomonas campestris pv vesicatoria infection, line 50 only slightly, and all mutant lines show symptoms delayed, expression of senescent-associated genes is decreased, mutants have about 50% lower starch content due to 2-fold increased sucrose export, and show a tendency for increased fructose content | Solanum lycopersicum | ? | - |
? | |
3.2.1.26 | sucrose + H2O | - |
Solanum lycopersicum | D-fructose + D-glucose | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.26 | cell wall-bound invertase | - |
Solanum lycopersicum |