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Literature summary extracted from

  • Sansom, F.M.; Riedmaier, P.; Newton, H.J.; Dunstone, M.A.; Mueller, C.E.; Stephan, H.; Byres, E.; Beddoe, T.; Rossjohn, J.; Cowan, P.J.; dApice, A.J.; Robson, S.C.; Hartland, E.L.
    Enzymatic properties of an ecto-nucleoside triphosphate diphosphohydrolase from Legionella pneumophila: substrate specificity and requirement for virulence (2008), J. Biol. Chem., 283, 12909-12918.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.5 expression of a His6-tagged Lpg1905 using plasmid pRSET:lpg1905 in Escherichia coli BL21(DE3) C41 strain Legionella pneumophila

Protein Variants

EC Number Protein Variants Comment Organism
3.6.1.5 E159A site-directed mutagenesis, inactive mutant Legionella pneumophila
3.6.1.5 additional information following intratracheal inoculation of A/J mice, none of the Lpg1905 mutants is able to restore virulence to an lpg1905 mutant during lung infection Legionella pneumophila
3.6.1.5 N168A site-directed mutagenesis, the mutant shows reduced activity due to decreased affinity for the nucleotide substrates, with a relatively increased Km 1.3fold for ATP hydrolysis and 3fold for ADP hydrolysis for the mutant enzyme, the mutant partially restores the ability of an enzyme-deficient Legionella pneumophila lpg1905 mutant strain to replicate in THP-1 macrophages Legionella pneumophila
3.6.1.5 Q193A site-directed mutagenesis, inactive mutant Legionella pneumophila
3.6.1.5 R122A site-directed mutagenesis, inactive mutant Legionella pneumophila
3.6.1.5 W384A site-directed mutagenesis, inactive mutant Legionella pneumophila

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.5 POM-1 i.e. Na6[H2W12O40], a polyoxometalate Legionella pneumophila
3.6.1.5 POM-6 i.e. (NH4)18[NaSb9W21O86], a polyoxometalate Legionella pneumophila

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.5 additional information
-
 additional information substrate specificity and Michaelis-Menten kinetics, overview Legionella pneumophila
3.6.1.5 0.4
-
 ATP pH 7.4, 37°C, recombinant enzyme Legionella pneumophila
3.6.1.5 1
-
 ADP pH 7.4, 37°C, recombinant enzyme Legionella pneumophila

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.5 Ca2+ activates Legionella pneumophila
3.6.1.5 Mg2+ activates Legionella pneumophila
3.6.1.5 additional information Lpg1905 is dependent on divalent metal cations Legionella pneumophila
3.6.1.5 Zn2+ activates Legionella pneumophila

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.5 additional information Legionella pneumophila Lpg1905 is essentially required for intracellular replication of Legionella pneumophila in eukaryotic cells leading to the Legionnaires disease, a severe and potentially fatal form of pneumonia ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.5 Legionella pneumophila
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.5 recombinant His6-tagged Lpg1905 from Escherichia coli BL21(DE3) C41 strain by nickel affinity chromatography Legionella pneumophila

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.6.1.5 additional information
-
 catalytic efficiency Legionella pneumophila

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.5 ADP + 2 H2O
-
 Legionella pneumophila adenosine + 2 phosphate
-
 ?
3.6.1.5 ATP + 2 H2O slight preference for ATP as substrate Legionella pneumophila AMP + 2 phosphate
-
 ?
3.6.1.5 GDP + H2O
-
 Legionella pneumophila GMP + phosphate
-
 ?
3.6.1.5 GTP + 2 H2O
-
 Legionella pneumophila GMP + 2 phosphate
-
 ?
3.6.1.5 additional information Lpg1905 is essentially required for intracellular replication of Legionella pneumophila in eukaryotic cells leading to the Legionnaires disease, a severe and potentially fatal form of pneumonia Legionella pneumophila ?
-
 ?
3.6.1.5 additional information the enzyme shows the ability to hydrolyze nucleoside tri- and diphosphates, but has limited activity against CTP, CDP, UTP, and UDP Legionella pneumophila ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.1.5 More the enzyme contains five apyrase conserved regions Legionella pneumophila

Synonyms

EC Number Synonyms Comment Organism
3.6.1.5 ecto-nucleoside triphosphate diphosphohydrolase
-
 Legionella pneumophila
3.6.1.5 Lpg1905
-
 Legionella pneumophila
3.6.1.5 More Lpg1905 is a prokaryotic member of the CD39/NTPDase1 family of enzymes Legionella pneumophila
3.6.1.5 NTPDase
-
 Legionella pneumophila

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.6.1.5 37
-
 assay at Legionella pneumophila

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.5 7.4
-
-
 Legionella pneumophila

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.6.1.5 5 9 significant hydrolysis of both ATP and ADP at pH 6.5-pH 8.0, negligible hydrolysis of ATP or ADP substrates occurs at pH 9.0 or at pH 6.0 or lower Legionella pneumophila

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.6.1.5 0.067
-
 POM-6 pH 7.4, 37°C, recombinant enzyme Legionella pneumophila
3.6.1.5 0.267
-
 POM-1 pH 7.4, 37°C, recombinant enzyme Legionella pneumophila