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Literature summary extracted from

  • Kumar, A.K.; Goswami, P.
    Dissociation and reconstitution studies of a broad substrate specific multimeric alcohol oxidase protein produced by Aspergillus terreus (2009), J. Biochem., 145, 259-265.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.3.13 DMSO complete inhibition by 50% DMSO Aspergillus terreus
1.1.3.13 additional information no inhibition by ethylene glycol, even at high concentrations Aspergillus terreus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.3.13 membrane
-
 Aspergillus terreus 16020
-
1.1.3.13 microsome
-
 Aspergillus terreus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.13 269000
-
 native enzyme, gel filtration Aspergillus terreus

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.13 Aspergillus terreus
-
-
-
1.1.3.13 Aspergillus terreus MTCC 6324
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.13 native enzyme by microsomal membrane preparation by differential centrifugation. The enzyme is solubilized from the microsomal membrane using 0.5% (v/v) 3-[(3-cholamidopropyl) dimethyl ammonio] propane-1-sulfonic acid, i.e. CHAPS, and further purified by ammonium sulfate fractionation followed by anion exchange chromatography Aspergillus terreus

Renatured (Commentary)

EC Number Renatured (Comment) Organism
1.1.3.13 enzyme dissociation by 2 M KBr in 0.25 M potassium phosphate, pH 7.5, 0.3 mM EDTA, 5 mM 2-mercaptoethanol and 20% glycerol, for 4 days at 4°C. The inactive dissociated enzyme protein is renaturated, but still catalytically inactive, by assembly after removal of 2-mercaptoethanol and incubation with cofactor FAD. The enzyme activity is regained by incubation with the substrate. The dissociation process traverses through two FAD-associated intermediate proteins, one of whhich shows the enzyme activity. The catalytic reactivation is a slow process. Dynamic light scattering analysis, overview. DMSO and ethylene glycol are ineffective in dissociating this alcohol oxidase Aspergillus terreus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.13 additional information
-
 substrate specificity of native, intermediate dissociated protein, and re-associated protein, overview. The re-associated enzyme protein shows poor activity with substrate (R)-2-octanol Aspergillus terreus
1.1.3.13 37
-
 native intermediate dissociated enzyme protein, substrate (R)-2-octanol Aspergillus terreus
1.1.3.13 42
-
 re-associated enzyme protein, substrate n-dodecanol Aspergillus terreus
1.1.3.13 45
-
 re-associated enzyme protein, substrate phenyl-3-propanol Aspergillus terreus
1.1.3.13 49
-
 native intermediate dissociated enzyme protein, substrate n-dodecanol Aspergillus terreus
1.1.3.13 62
-
 native enzyme, substrate n-dodecanol Aspergillus terreus
1.1.3.13 63
-
 native enzyme, substrate (R)-2-octanol Aspergillus terreus
1.1.3.13 96
-
 re-associated enzyme protein, substrate n-heptanol Aspergillus terreus
1.1.3.13 183
-
 native intermediate dissociated enzyme protein, substrate phenyl-3-propanol Aspergillus terreus
1.1.3.13 190
-
 native intermediate dissociated enzyme protein, substrate n-heptanol Aspergillus terreus
1.1.3.13 250
-
 native enzyme, substrate phenyl-3-propanol Aspergillus terreus
1.1.3.13 305
-
 native enzyme, substrate n-heptanol Aspergillus terreus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.13 (R)-2-octanol + O2
-
 Aspergillus terreus octan-2-one + H2O2
-
 ?
1.1.3.13 (R)-2-octanol + O2
-
 Aspergillus terreus MTCC 6324 octan-2-one + H2O2
-
 ?
1.1.3.13 3-phenylpropan-1-ol + O2
-
 Aspergillus terreus 3-phenylpropanal + H2O2
-
 ?
1.1.3.13 3-phenylpropan-1-ol + O2
-
 Aspergillus terreus MTCC 6324 3-phenylpropanal + H2O2
-
 ?
1.1.3.13 additional information substrate specificity of native, intermediate dissociated protein and re-associated protein, overview Aspergillus terreus ?
-
 ?
1.1.3.13 additional information substrate specificity of native, intermediate dissociated protein and re-associated protein, overview Aspergillus terreus MTCC 6324 ?
-
 ?
1.1.3.13 n-dodecanol + O2
-
 Aspergillus terreus n-dodecanal + H2O2
-
 ?
1.1.3.13 n-dodecanol + O2
-
 Aspergillus terreus MTCC 6324 n-dodecanal + H2O2
-
 ?
1.1.3.13 n-heptanol + O2
-
 Aspergillus terreus n-heptanal + H2O2
-
 ?
1.1.3.13 n-heptanol + O2
-
 Aspergillus terreus MTCC 6324 n-heptanal + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.3.13 multimer the enzyme is composed of several subunits of of 85, 63, 43, 27 and 13 kDa, the 27 kDa subunit is degraded to 25 kDa, overview Aspergillus terreus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.3.13 30
-
 assay at Aspergillus terreus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.13 FAD essential Aspergillus terreus